BIOCHEMISTRY EXAM 2 | QUESTIONS & ANSWERS (VERIFIED) | LATEST UPDATE | GRADED A+

BIOCHEMISTRY EXAM 2 | QUESTIONS & ANSWERS (VERIFIED) | LATEST UPDATE | GRADED A+ Covalent Catalysis Correct Answer: Active site contains a reactive group (Nucleophile) General Acid-Base Catalysis Correct Answer: Molecule (other than Water) acts as a proton donor or acceptor Metal Ion Catalysis Correct Answer: Metal ion serves as an electrophilic catalyst and stabilizes reaction intermediate Temperature Correct Answer: As temperature rises, reaction rate increases; loss of activity at high temperature pH Correct Answer: Activity of most enzymes is bell-shaped; optimal pH varies with enzyme 2 Reversible Enzymes Correct Answer: Rapid dissociation of enzyme-inhibitor complex; 3 types: competitive, uncompetitive, noncompetitive Competitive Inhibition Correct Answer: Inhibitor resembles substrate and binds to active site of enzyme Uncompetitive Inhibition Correct Answer: Inhibitor binds only to enzyme-substrate complex and cannot be overcome by adding more substrate Noncompetitive Inhibition Correct Answer: Inhibitor and substrate can bind simultaneously to enzyme, decreases overall number of active enzymes Irreversible Inhibition Correct Answer: Inhibitor dissociates very slowly from target enzyme, tightly bound covalently or non-covalentl