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MICROBIOLOGY LAB PRACTICAL 1 VERIFIED RATIONALES LATEST 2024/2025

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Gram Stain Positive: Purple Negative: Pink 1. 4-5 layers 2. Crystal Violet 3. Iodine 4. Decolorizer 5. Safrin 2. Bacteria Shape Bacillus: rod shaped Coccus: round shape Spiral: spiral shaped 3. Catalase Test Hydrogen peroxide is toxic to bacteria and may be produced in the cell. Some bacteria contain catalase which can detoxify the hydrogen peroxide by converting it to water and gaseous oxygen. Positive: Bubbles Negative: Nothing happens Oxygen gas comprises the bubbles 4. Oxidase Test Some cytochrome containing orga

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