Advanced Biochem ACS Exam 2024 Questions with correct Answers

Advanced Biochem ACS Exam 2024 Questions with correct Answers Coenzymes required by the pyruvate dehydrogenase multienzyme complex are - answerThiamine pyrophosphate, lipoic acid, Fad, CoA, & NAD+ A kinase is an enzyme that... - answertransfers a phosphoryl group from ATP Which of these contains a thioester bond: - answersuccinyl CoA In setting up an assay for measuring the total amount of an enzyme present in an unknown sample, the substrate concentration should preferably be: - answer10 fold higher than Km Dialysis would be most useful for the separation of: - answerA ammonium sulfate from an enzyme An inhibitor that increases Km and does not alter the V max value is - answerA competitive inhibitor Which amino acid side chain would not be expected would not be expected to participate in covalent enzyme catalysis - answerLeu Which statement is correct concerning the T(deoxy) and R(oxy) states of tetrameric hemoglobin - answerIn the T to R transition, the central cavity of the tetramer decreases in size, preventing the binding of the allosteric modulator 2,3,-biophosphylglycerate When the energy needs of the cell are satisfied, acetyl-CoA accumulates and serves as a positive allosteric effector for - answerpyruvate carboxylase To determine the Km value for an enzyme reaction, which parameter is normally varied, while keeping the others constant - answersubstrate concentration In a yeast cell growing aerobically on glucose, which would provide most of the energy for growth? - answerglycolysis to TCA cycle and oxidative phosphorylation Cleavage of disulfide bonds in globular proteins may be brought about by - answerReaction with excess B-mercaptoethanol at pH 8 in 6 M urea The alpha helical secondary structure found in protein - answeris disrupted by proline, amine H and carbonyl O