MB (ASCP) Exam Questions and Answers Graded A

MB (ASCP) Exam Questions and Answers Graded A Predominant form of DNA - Answer ️️ -Right-handed B DNA and histone interaction is due to what type of bond? - Answer ️️ -Ionic What stains to the minor groove of DNA duplex? - Answer ️️ -SYBR Green What is the most abundant type of RNA? - Answer ️️ -Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? - Answer ️️ -Genome and mRNA What increases half-life of mRNA? - Answer ️️ -5' Methyl Cap What kind of DNA is most likely to be transcriptionally active? - Answer ️️ -Euchromatin What is the most common type of eukaryotic DNA? - Answer ️️ -Satellite DNA What specimen has the most DNA according to molecular weight? - Answer ️️ -White Blood Cells What amino acids compose histones? - Answer ️️ -Lysine and Arginine Amino acid that all proteins start with: - Answer ️️ -Methionine What protects the DNA strand during replication? - Answer ️️ -Single-stranded binding protein Leading Strand - Answer ️️ -3' to 5' template Lagging Strand - Answer ️️ -5' to 3' template What DNA strand is being copied in transcription? - Answer ️️ -Antisense Strand First step after newly formed mRNA is made? - Answer ️️ -Addition of poly(A) tail Every time the chromosome is copied it would become shorter if not for what enzyme? - Answer ️️ -Telomerase What polymerase has repair activity? - Answer ️️ -Beta/Epsilon What polymerase acts on leading strand? - Answer ️️ -Delta What polymerase acts on lagging strand? - Answer ️️ -Alpha Which polymerase is used for mitochondrial replication and repair? - Answer ️️ -Gamma What polymerase binds the 5' end to 3' end of dsDNA? - Answer ️️ -Topoisomerase Which RNA polymerase is mainly for transcription? - Answer ️️ -RNA Pol II Which polymerase is involved with mRNA synthesis? - Answer ️️ -RNA Pol II What inactivates RNAses? - Answer ️️ -Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours at 400C What type of restriction enzyme recognizes palindromic sites? - Answer ️️ -Restriction Enzyme Type II What type of restriction enzyme lacks methylation activity? - Answer ️️ -Restriction Enzyme Type II What is necessary to make cDNA? - Answer ️️ -Reverse transcriptase Number of SNPs in a human genome? - Answer ️️ -11 billion (10^7) How many genes (base pairs) are in a human haploid genome? - Answer ️️ -3 billion base pairs The polymerization of acrylamide requires: - Answer ️️ -Catalyst (APS or TEMED) or Light activation How does agarose gel pore size relate to concentration? - Answer ️️ -Inversely proportional What is denatured during a southern blot? - Answer ️️ -Hydrogen bonds (inter-strand bonds) Melting temperature formula - Answer ️️ -Tm = (4C x GC Pairs) + (2C x AT pairs