Microbiology Chapter 9 Exam Questions with 100% Correct Answers | Verified | Updated

Microbiology Chapter 9 Exam Questions with 100% Correct Answers | Verified | Updated B - answer1) The following steps are used to make DNA fingerprints. What is the third step? A) Collect DNA. B) Digest with a restriction enzyme. C) Perform electrophoresis. D) Lyse cells. E) Add stain. B - answer2) How many pieces will EcoRI produce from the plasmid shown in Figure 9.1? A) 1 B) 2 C) 3 D) 4 E) 5 D - answer3) In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the ampicillin-resistance (amp) gene? A) 0.17 kilobase pairs B) 0.25 kbp C) 1.08 kbp D) 1.50 kbp E) 3.00 kbp C - answer4) In Figure 9.2, the enzyme in step 1 is A) DNA polymerase. B) DNA ligase. C) RNA polymerase. D) Reverse transcriptase. E) Spliceosome. A - answer5) In Figure 9.2, the enzyme in step 2 is A) DNA polymerase. B) DNA ligase. C) RNA polymerase. D) Reverse transcriptase. E) Spliceosome. B - answer6) The reaction catalyzed by DNA polymerase is A) DNA → mRNA B) mRNA → cDNA C) mRNA → protein D) DNA → DNA E) tRNA → mRNA C - answer7) Which of the following is an advantage of using E. coli to make a human gene product? A) Endotoxin may be in the product. B) It doesn't secrete most proteins. C) Its genes are well known. D) It can't process introns. E) None of the above. E - answer8) Which of the following is NOT an agricultural product made by DNA techniques? A) Frost retardant B) Bacillus thuringiensis inse