Microbiology Lab Final Exam Questions and Answers

Microbiology Lab Final Exam Questions and Answers agent that kills bacteria upon exposure -Answer-bacteriocidal agent naturally produced by microorganisms -Answer-antibiotic agent that slows the growth of bacteria -Answer-bacteriostatic agent artificially synthesized or manipulated from other products -Answer-antimicrobial targets DNA gyrase, preventing DNA replication within a cell -Answer-novobiocin Disrupts cell wall stability, eventually leading to cell lysis -Answer-penicillin binds with bacterial ribosomes to prevent cell growth and cell division -Answer- gentamicin the mold that alexander fleming observed preventing bacterial growth was -Answer- penicillium notatum since the discovery and use of antibiotics, we no longer need to worry about bacterial infections -Answer-false penicillin works by -Answer-disrupting cell wall stability novobiocin prevents the function of ______ in a cell -Answer-DNA gyrase bacteriostatic antibiotics cause an inhibition of growth rather than cell lysis and death - Answer-true gentamicin binds with bacterial _______ -Answer-ribosomes the effective zone of inhibition is the same for all antibiotics -Answer-false an antibiotic is a synthetic drug produced in a laboratory -Answer-false ______ is a genus that produces antibiotics -Answer-all of the above: Bacillys, Cephalosporium, and Streptomyces penicillin is produced by a ____ -Answer-mold novobiocin is a bacteriostatic, narrow-spectrum antibiotic -Answer-true gentamicin is synthesized by a ___ -Answer-gram-positive bacteria bacteriocidal agents kill bacteria by _____ -Answer-cell wall lysis resistant bacteria will exhibit ____ during a kirby-bauer diffusion test -Answer-no zone of inhibition a spreader is used when inoculating a kirby-bauer diffusion plate to ___ -Answer-create a uniform bacterial lawn zones of inhibition should be measured after they exceed 25 mm -Answer-false the diagram below illustrates resistance to drug ___ (nothing around A, little around B, large around C) -Answer-A ______ are asexually produced spores of the penicillum fungus -Answer-conidia describes individual colonies on a plate's surface -Answer-colony forming unit the number of individuals doubles each generation -Answer-exponential growth cloudiness of a fluid caused by large numbers of particles/organisms -Answer-turbidity the measure of the light intensity absorbed by a sample -Answer-absorbance asexual reproduction resulting in two daughter cells -Answer-binary fission order the steps for performing a dilution series -Answer-1. determine the dilutions of the dilution tubes 2. determine which plate is the countable plate 3. calculate the concentration in the original tube binary fission is a method of sexual reproduction where a single cell (parent cell) will split into four cells (daughter cells) -Answer-false plates with ____ colonies per plate are used to calculate CFUs -Answer-30-300