Parasitology- Exam Questions With 100% Correct Answers 2024

Parasitology- Exam Questions With 100% Correct Answers 2024 How do we find and identify parasites - answerMicroscopy, organism detection, antibody detection, antigen detection PVA - answerpolyvinyl alcohol, keep organism intact until specimen can be examined. Used to make permanent smears, help specimen adhere to slide. doesn't work with immunoassay methods. SAF - answerSodium Acetate Formalin, permanent smears and concetrations. doesnt adhere as well, slides dont last as long. can be used with immunoassay kits. Contains more fecal debris than floatation methods macroscopic examination - answerareas with mucus or blood direct wet mount - answerdetect motile forms iodine staining - answerdifferentiate parasites Concentration methods - answerFlotation: mixes stool specimen with solution high in specific gravity, debris settle to bottom, cysts and eggs float to the top Sedimentation:All parasites will sediment if you centrifuge the specimen. Centrifugation in formalin-ethyl acetate extracts some of the fecal debris, and also kills and preserves the parasites most commonly used stain for parasites - answerWheatley trichrome stain. Debris=green, cytoplasm=blue/green/lavender. Nuclei=red/purple Protozoa - answerone-celled organisms. move via pseudopods Two life stages of protozoa - answer1. Trophozoites - growth stages, motile 2. Cysts - nonmotile, resistant to environment, infectious stage treatment for protozoa - answermetronidazole types of intestinal protozoans - answeramoebae, flagellates, ciliate, coccidia amebic dysentery - answerdiarrhea contains blood and/or mucus. Acquire by ingestion of cysts in food or water.. common in tropical areas with poor sanitation. Only E. histolytica is considered pathogenic