Histotechnology - Fixation Question and answers already passed 2023/2024

Histotechnology - Fixation Question and answers already passed 2023/2024 What are the steps tissue must go through before it is viewed under a microscope? - correct answers 1. Fixation 2. Processing 3. Embedding 4. Microtomy 5. Staining 6. Coverslipping Define fixation. - correct answers The preservation of cells and tissue in as life-like a way as possible by stabilizing the protein so that it is resistant to further changes. What are the functions of fixation? - correct answers 1. To prevent putrefaction and autolysis 2. To help maintain proper relationships b/w cells and extracellular substances 3. To bring out diff's in refractive indexes, and to inc. visibility of or contrast b/w diff tissue elements 4. To enhance staining 5. To make cell constituents insoluble (spec. proteins) 6. To prevent dessication 7. To prevent osmotic swelling/shrinkage