HTL Histotechnology Special Stains Question and answers rated A+ 2023/2024

HTL Histotechnology Special Stains Question and answers rated A+ 2023/2024This is a stain for acid mucopolysaccharides and sialomucins. - correct answers Alcian blue This stains reacts with cations and is used to find calcium in tissue. - correct answers Alizaran Red S This stains uses ferric ions to demonstrate mucopolysaccharides and glycoproteins. Must be done at a low pH. - correct answers Colloidal iron In this stain, tissue is first treated with alkali to open up binding sites through the release of hydrogen bonds. - correct answers Congo Red This stain,combined with HCl and paraldehyde, stains elastic fibers and beta cells a deep purple. - correct answers Gomori's Aldehyde Fuschin The first step of this silver stain is the oxidation of hexose sugars into aldehydes by potassium permanganate. - correct answers Gomori's Stain for Reticulin Fibers In this stain, a plasma stain and a CT fibers stain are combined with phosphotungstic acid and acetic acid. Demonstrates collagen and muscle. - correct answers Gomori's Trichrome This stain combines a PAS stain, Weigert's hematoxylin, and a selective collagen stain combining picric acid and methyl blue. - correct answers Lillie's Allochrome This stain uses tungsten in phosphotungstic acid to bind any available hematein, giving a blue lake. - correct answers Mallory's PTAH This is a sequence procedure combining Biebrich scarlet, phosphotungstic or phosphomolybdic acid, and aniline blue. - correct answers Masson's Trichrome