BIOD171 Microbiology Lab Notebook 2 2 1 5

BIOD171 Microbiology Lab Notebook 2 2 1 5 Psychiatric/Mental Health Nursing (Santa Fe College) Studocu is not sponsored or endorsed by any college or university Portage Learning BIO 171- Microbiology Lab Notebook Lab Notebook Bookmarks (click to navigate): Lab 1 Notebook Lab 2 Notebook Lab 3 Notebook Lab 4 Notebook Lab 5 Notebook Lab 6 Notebook Lab 7 Notebook Lab 8 Notebook Lab 9 Notebook 1 Lab 1 Notebook Back to Home Page Title: KD01 Introduction to medical microbiology & lab notebook procedures Course Objectives: Cultivation of samples: growth conditions and equipment used Identification of samples: biochemical assays & tests available Evaluation of samples: microscopy- visualization and key characteristics of specimens Basic Equipment: Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take minutes to sterilize & hot dry air which will take hours; before opening make sure it is depressurized and will have very hot air. Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes - Shaker incubator 37°C shakes culture & rotates to aerate- liquid nutrient broth Visualizing: microscopy Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term Lab Safety 1. Never eat or drink in the lab: contamination risks 2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue, green),thermal, or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills 2 3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria Outline of how to set up Notebook Objective: To establish an organized template for keeping experimental records, procedures, and results. Procedure: 1. This is where each step of a given protocol is recorded 2. Be sure to clearly indicate any deviations that may occur during the experiment- put them in red Notes: Additional (helpful) information placed here Results: A summary of the final outcome of the experiment should be described here 3 Lab 2 Notebook Back to Home Page Title: KD02 Introduction to Microscopy Objective: To learn basic parts of microscope and how to view a sample Procedure: 1. Review the parts of microscope 2. Load sample to be viewed 3. Choose magnification 4. Adjust microscope so the sample is clear Notes: Parts of microscope: 1. eye pieces (ocular lens): can be pulled apart; should be able to use both eyes and see one circle if not adjust 2. arm/neck: if moving this is what you will grab with one hand and hand under base 3. Objective lenses: this provides the magnification of the sample; shortest has the least magnification (4x, 10x, 40x,100x) 4. Stage: the flat surface that you place your sample; holds the sample via a clamp (squeeze together to place sample and release); stage clips raise up and lay on the glass coverslip 4 5. Focus knobs: located on the side of the arm/neck; Outside ring is the coarse adjustment – makes large steps in focus; Inner ring is the fine focus- you can see the specimen but need more detail. 5 6. Iris diaphragm: located below the stage determines how much light passes through the sample (the lever slides) 7. Base- the bottom of the microscope and should be steady and on flat surface Load sample to be viewed: 1. Put sample on stage and make sure it is secured 2. Turn on. Left hand side you can dim or brighten the light. You can use either the knob or the diaphragm to control the light. Types of Objectives: dry vs oil (required on higher magnification) add a drop of oil onto slide- helps with light refraction and lense will be embedded in the oil 3. Intensity of light source: too bright = saturation (can’t see); too dark = low visibility. Start midway leave iris open 4. Stage guides below stage 2 knobs: top controls movement of the stage forward/backward. Lower know moves left and right. These put specimen in range of viewing. 5. *if unsure of magnification: start on lowest power Power of Objective X Power of Eyepiece = Total magnification: 15 mm diameter object & total magnification is 200x larger and the diameter is 3000mm Eye pieces are labeled with magnification on them and are removable Coarse adjustment will raise or lower the stage- do not touch the sample with the lenses. Look through eyepiece and slowly make adjustments. There will be a pointer inside the eye piece you can spin the eye piece and the line will point to your sample