Molecular Diagnostics Chapter 2 Exam Graded A+

Molecular Diagnostics Chapter 2 Exam Graded A+ Transcription - answersynthesis of an RNA molecule from a DNA template RNA polymerase - answerenzyme that links together the growing chain of RNA nucleotides during transcription using a DNA strand as a template, occurs during metaphase Which RNA polymerase synthesize noncoding RNA? - answerPol I and III Promoter - answerspecific region of a gene where RNA polymerase can bind and begin transcription Are there more RNA polymerase or DNA polymerase in the cell? - answerRNA polymerase Does the antisense strand or sense strand if the DNA template have an identical sequence to that of the RNA product? - answerthe sense strand has an identical sequence to the mRNA (except for T-->U sub.), the antisense strand is used as the template What are the three steps of mRNA capping? - answer1. hydrolysis of the 5' phosphate 2. transfer of a guanine monophosphate 3. methylation of the attached guanine in the N7 position What is the purpose of the cap on mRNA? - answerprotects the cap during transcription and facilitates ribosome binding during translation Rho - answertranscription factor that inactivates the elongation complex at the site of termination In eukaryotes when does transcription end? - answerat the poladenylated site which activates termination of transcription