Uark microbiology lab practical 1 rated A

Uark microbiology lab practical 1 rated A Coccus A spherical bacterium. Bascillus Rod shaped bacteria Spirillum A spiral-shaped bacterium. Uses of good bacteria on our bodies, in yogurt, and can be used to clean up oil and radiation colony visible mass of cells Innoculation putting bacteria into a medium what is the purpose of aseptic technique -no contaminating organisms are introduced into culture materials during handling or inoculation -handler and other people are not contaminated by organisms -no contamination remains after you have worked with cultures b cereus endospore stain green: endospore pink: vegetative endospore resting caused by lack of nutrients endospore resistance resistance to heat, radiation, acids, chemicals, and cell decication what kind of stain is endospore stain special stain exosporium protein coat of endospore ocular eyepiece endospore stain and coutnerstain -malachite green -counterstain: safranin mycolic acid Waxy lipid, makes cells not permeable to most stains. primary stain in acid fast is Carbolfuchsin: contains phenol which the makes the membrane permeable secondary stain in acid fast is methylene blue Why are the cells called acid fast? the decolorizer acid-alcohol does not remove the trap stain from their cells bacteria in acid fast stain mycobacterium and stapholococcus color of mycobacterium in acid fast stain pink because they are acid fast color of staphylococcus in acid fast stain methylene blue makes them blue Mycobacterium smegmatis acid fast stain The acid-fast Mycobacterium retains carbol fuchsin and stains hot pink. The Staphylococcus epidermidis is decolorized and the counterstain colors them blue. Pink rods = Acid-fast Blue cocci = nonacid-fast 3 bacteria in the pure culture technique 1. ecoli: white; fecal matter 2. pseudomonas aeruginosa: green; found in the environment and is an issue 3. staphylococcus aureus; yellow; found on the skin Nosepiece Holds the objectives and can be rotated to change the magnification Good smears are important to observe - morphology - arrangement - internal structures Two types of morphology bacilli: rods cocci: spherical Two types of arrangements staph: cluster strep: chain smear prep staphylococcus and bacillus gram staining invented by christian gram to identify between bacteria and eukarya cells gram staining primary stain crystal violet gram staining mordant stain iodine gram staining decolorization alcohol gram positive thick layer of peptidoglycan (purple) gram negative thin layer of peptidoglycan (pink) monotrichous flagella one flagella lophotrichous flagella cluster of flagella at one end peritrichous flagella spread over entire surface of cell amphitrichous flagella flagella at both ends Brownian motion movement caused by currents under the cover glass Excersise 6: Which one is motile? B cerrius is motile and klebsiella is magnifications 10x,40x,100x condenser located under the stage collects and directs the light from the lamp to the slide being studied diaphragm (within condenser) Regulates the amount of light on the specimen coarse adjustment knob and fine adjustment knob sides of microscope and used to bring objects into focus (up and down) primary stain crystal violet mordant stain iodine decolorization alcohol counterstain safranin when viewed by electron microscopy, gram + cells have what type layer of peptidoglycan? thick when viewed by electron microscopy, gram - cells have what type layer of peptidoglycan? outer membrane that covers a much thinner layer After the crystal violet and iodine have been added to the slide both gram +/- are purple After alcohol has been added to the slide gram + stays purple, but gram - is colorless After safranin is added to the slide gram + remains purple, gram - turns pink Christian Gram developed gram stain How long do we store pure culture? 48hours At what temperature do we store pure culture? 37 degrees C How many times do you go through a flame for heat fixing? 3 times Lab 5 Bacteria S. aureus P. aeruginosa E. coli M. catarrhalis Lab 6 Bacteria B. cereus K. pneumoniae P. vulgaris M. luteus Lab 7 Bateria S. aureus E. coli P. aeruginosa Lab 8 Bacteria B. megaterium Lab 9 Bacteria M. smegmatis S. aureus