Microbiology - Lab Practical 1 100% Correct

Microbiology - Lab Practical 1 100% Correct Ocular Lens(es) Eyepiece Magnifies 10X Objective Lens(es) Parfocal Usually 4X, 10X, 40X, 100X Mechanical Stage Allows you to move a slide in order to see different specimens on it Condenser Focuses light from light source Iris Diaphram Controls amount of light that passes through a specimen Coarse Adjustment (Knob) Helps to focus image Should never be used with 100X lens Fine Adjustment (Knob) Helps to focus image details Resolution The ability of a microscope to view two objects as separate units Total Magnification The magnification of the objective lens multiplied by the magnification of the ocular lens Refraction The bending of light as it passes through objects with different densities Immersion Oil Oil used at 100X magnification to combat refraction Resolving Power A measure of the clarity of the image; it is the minimum distance two points can be separated and still be distinguished as two separate points Numerical Aperture The characteristic of a lens which determines the amount of light that enters the lens. The higher the numerical aperture, the smaller the minimum resolvable distance and the better the resolution Real Image An image produced in an optical device, such as a lens or mirror, that is formed by converging rays. Virtual Image An image formed through reflection or refraction that can be seen by an observer but cannot be projected on a screen because light from the object does not actually come to a focus. Refractive Index A measure of the light-bending ability of a medium Binocular Two ocular lenses Monocular One ocular lens 4X 10X