Molecular Diagnostics EXAM GRADED A+

Molecular Diagnostics EXAM GRADED A+ If 20% of the nucleotides in an organism are adenine, predict % of guanine - answer30% NOT required for DNA replication by PCR - answerDNA ligase 1st letter of restriction endonucleases name is the - answerbacterial genus enzyme recognizes 5'CT ^ ATAG 3' and cuts as indicated. Predict ends of the complementary DNA strand - answer3'GATA5' // 3'TC5' The abs of a 1:100 dilution of isolated dsDNA solution, measured at 260nm is .062. Reasonable estimate for the dsDNA concentration of sample expressed as ug/mL - answer310 (100x.63x50) in the isolation of RNA diethylpyrocarbonate (DEPC) is used to - answerinhibit RNase purification resins used to isolate DNA take advantage of fact DNA is - answernegatively charged after DNA electrophoresis the isolated bands in the kilobase size range appear too close together. What can be done to improve appearance and seperation of bands in sample - answerincrease the running time of the electrophoresis assay commonly used as label in molec diag - answerBiotin NOT an example of target amplification - answerBranched chain DNA amplification (bDNA) in forensic testing, DNA fingerprinting can ID individuals w high accuracy bc - answerhuman loci are polymorphic