(MB) ASCP Practice Exam Questions and Answers Solved Correctly

(MB) ASCP Practice Exam Questions and Answers Solved Correctly Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base - Answer ️️ -Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: - Answer ️️ -Thymine What genes would be screened in a breast cancer panel? - Answer ️️ -HER2, ERBB2, BRCA1 Next Generation Sequencing uses: - Answer ️️ -Short sequence reads Purines and pyrimidines differ from each other in that: - Answer ️️ -Purines have two rings; pyrimidines have one ring The purines are: Cytosine and uracil Adenine and thymine Thymine and cytosine Adenine and guanine - Answer ️️ -Adenine and guanine What is the rate of mutation per round of DNA replication? 1 in 1,000 base pairs 1 in 10,000 base pairs 1 in 1,000,000 base pairs 1 in 1,000,000,000 base pairs - Answer ️️ -1 in 1,000,000,000 base pairs The rate of DNA migration through an agarose gel during electrophoresis does not depend on which of the following factors? Net charge of the molecule Size of the molecule Shape of the molecule Nucleotide sequence of the molecule - Answer ️️ -Nucleotide sequence of the molecule What are the phases in a qPCR Amplification Plot? Initiation, exponential, plateau Baseline, exponential, plateau Baseline, threshold, exponential, plateau Baseline, initiation, threshold, exponential, plateau - Answer ️️ -Initiation, exponential, plateau Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'? 5'-GAC 3'-GAC 3'-CTG 5'-GTC - Answer ️️ -3'-GAC A patient with impaired judgment, personality changes, signs of abnormal body movements and depression comes to the physician's office for a follow-up visit. The physician suspects a single- gene disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your clinical laboratory for mutation screening in the Huntington gene. Testing with standard PCR indicates that patient has Huntington Disease, HD. Which of the following would be consistent with this diagnosis? 25 CAG repeats in the Huntington g