MIP 300 Final exam questions fully solved graded A+ 2023/2024

MIP 300 Final examwho provides the lipids in a viral envelope - correct answer the cell all viruses have a protein coat - correct answer true a virulent phage can inadvertently package host cell chromosome into phage particles - correct answer true you have a bacterium that picks up a new gene by Hfr conjugation. what is an explanation of why progeny of this bacterium express this trait? - correct answer the gene is integrated into the genome after successful transformation of a fragment of chromosomal DNA, the recipient (female) will become a donor (male) capable of creating a sex pili to transfer DNA - correct answer false you isolate a virulent page. It can perform specialized transduction - correct answer false you want to know if a baby still has vaccine neutralizing antibody that it received from its mom in utero. Which of the following types of ELISAs would you run? one that tests for - correct answer IgG in differential centrifugation, virus is first centrifuged at a slow speed, the supernatant is removed and re-centrifuged at a very fast speed - correct answer true Virus stocks prepared by differential centrifugation and gradient centrifugation are equally clean - correct answer false hemagglutination assay - correct answer determines highest dilution of virus that causes red blood cells to clump together cytopathic effect - correct answer a visible effect on a host cell, caused by a virus, that may result in host cell damage or death immuno fluorescence assay - correct answer primarily on biological samples and is classically defined as a procedure to detect antigens in cellular contexts using antibodies. plaque assay - correct answer method used to measure the number of viral particles present in a sample a temperate phage can inadvertently package host cell chromosome into phage particles - correct answer true you isolate a bacterium that can transfer genes to other bacteria only when it is in direct contact with these bacteria. it is most likely transferring the genes by - correct answer conjugation a piece of host cell dna bound to a piece of phage dna after excising from the chromosome. when this phage that contains host cell dna infects a new cell, will the new cell be killed by this infection? - correct answer no after successful generalized transduction, the recipient (female) will become a donor (male) capable of creating a sex pili to transfer dna - correct answer false can a temperate phage perform generalized and specialized transduction? - correct answer yes what kind of organism can carry out the conversion of O2 --> H2O? - correct answer aerobic respiration and aerobic chemolithotrophy an error in dna replication causes a mutation changing AGG to CGG, what change do you expect? - correct answer no change to protein function the organism with the more positive reduction potential will produce more energy - correct answer true dna ligase forms - correct answer the last phosphodiester bond between dna single strands