MIP 302 - Exam 2 questions with verified solution 2023/2024

MIP 302 - Exam 2 Where is the ocular lens located? - correct answer Where is the arm of the microscope located? - correct answer Where is the objective lens located? - correct answer Where is the diaphragm of the microscope located? - correct answer Where is the fine adjustment knob located? - correct answer Where is the coarse adjustment knob located? - correct answer Where is the light source of the microscope? - correct answer Where is the stage of the microscope? - correct answer Where is the base of the microscope? - correct answer How do you calculate total magnification? - correct answer Objective Magnification x Ocular Magnification = Total Magnification How do you calculate resolving power? - correct answer RP = (0.5 x Wavelength)/Numerical Aperture What is resolving power? - correct answer A microscope's ability to distinguish between two objects that are very close together What is the best resolving power for viewing microbes? - correct answer 0.2 um What is numerical aperture? - correct answer A lens' ability to gather light Explain aseptic technique. - correct answer Steps used to prevent microbial contamination - Wash your hands - Disinfect work bench - Work with open tubes near the flame - Never lay caps on the bench top - Work quickly How do you prepare a smear of cells for staining? - correct answer - Place a drop of DI water on the slide - Place a colony of bacteria on the water - Wait for the smear to air dry - Heat fix Why do you wait for smears to dry before heat fixing? - correct answer You don't want the cells to boil and burst How do you prepare a streak plate? - correct answer - Separate into 3 sections - Aseptically smear the bacteria in the first section then flame your loop - Pull twice from the first section into the second section then flame your loop - Pull twice from the second section into the third section then flame your loop How do you know if you've achieved isolation? - correct answer You are able to see individual colonies made up of the same sized, shape, and color bacteria How do you know if you achieved a pure culture? - correct answer There are no other colonies on the plate with different shapes, colors, or sizes