MIP 302 Final question n answers graded A+ 2023/2024

MIP 302 FinalPurpose of Blood Agar - correct answer See if bacteria has hemolysis abilities Purpose of Catalase Test - correct answer Determine if bacteria produces catalase Purpose of Indole Test - correct answer See if bacteria produces tryptophanase Medium for Indole test - correct answer Tryptone broth Purpose of Klinglers Iron Agar - correct answer See ability of bacteria to ferment glucose and/or lactose and the ability to produce CO2 and H2S gases Purpose of Lysine Decarboxylase Broth - correct answer Determine if bacteria produces Lysine Decarboxylase Purpose of MacConkey Agar - correct answer See if bacteria is gram negative and can ferment lactose Purpose of Methyl Red test - correct answer See if bacteria ferments glucose and produces mixed acids Medium from Methyl Red test - correct answer MRVP broth Purpose of Nitrate Broth - correct answer Distinguish bacteria based on their ability to reduce nitrate to nitrite Purpose of Ornithine Decarboxylase Broth - correct answer Determine if Bacteria produces Ornithine decarboxylase Purpose of Oxidase test - correct answer See if bacteria produces cytochrome oxidase Purpose of Phenol Red Carbohydrate Broth - correct answer See if certain bacteria ferment given carbs (sugars) Purpose of Phenylethyl Alcohol Agar - correct answer Isolate gram positive organisms from gram negative organisms Purpose of Pseudomonas Agar F - correct answer Differentiate bacteria based on their ability to produce Fluorescein (especially is Pseudomonas species) Purpose of Pseudosel Agar - correct answer Isolate and indentify Pseudosomas Aeruginosa Purpose of Simmons citrate Agar - correct answer See if bacteria can use citrate as sole source of carbon Purpose of Urea Slant - correct answer See if bacteria produces urease Purpose of Voges- Proskauer Test - correct answer See if bacteria can ferment glucose and produce acetoin Medium of Voges Proskauer Test - correct answer MRVP broth pH indicator used in KIA slants - correct answer Phenol Red pH indicator used in LDC broth - correct answer Bromocresol purple pH indicator used in MacConkey Agar - correct answer Neutral Red pH indicator used in Methyl Red test - correct answer Methyl Red pH indicator used in Ornithine Decarboxylase Broth - correct answer Bromocresol purple