ASCP Mock Exam | Questions with 100% Correct Answers | Verified | Latest Update 2024

The derived linear range for a spectrophotometer is 0.0550 to 1.270. The 260 OD absorbance reading for a sample is 1.35. What is the appropriate action? - dilute the sample slightly and repeat the quantitation Evaluation of a blood sample indicates the presence of a clot. Corrective action to salvage this specimen for analysis would most likely require - excess digestion of Proteinase K An advantage of nitrocellulose membranes is that - results of colormetric reactions are more intense When performing allele specific oligonucleotide procedures, which techniques are generally employed? - PCR, Autoradiography, Hybridization Which of the following actions should NOT be done to a nylon membrane containing DNA after a southern transfer? - Bake the membrane at 80C overnight Calf intestinal phosphatase is used during the cloning procedure to - prevent vector re-ligation Which of the following actions will bind DNA to a positively charged nylon membrane? - Baking at 80C for 2 hours Which of the following techniques is most appropriate for preparing high purity reagents for use in cloning procedures - sterilizing through a 0.25 um disposable Millipore filter