ASCP HT Exam Question and answer
100% solved 2023
To detect ferric iron in tissues. Diagnoses idiopathic hemochromatosis - correct answer
What is the purpose of the Prussian Blue stain?
Occupational Safety and Health Administration, a government agency in the
Department of Labor to maintain a safe and healthy work environment - correct answer
OSHA
Environmental Protection Agency
An independent federal agency established to coordinate programs aimed at reducing
pollution and protecting the environment - correct answer EPA
Federal agency that recommends ways to prevent work-related injury. Part of the CDC
and works with OSHA. - correct answer NIOSH
Center for Disease Control - correct answer CDC
College for American Pathologist. Lab accrediting agency. - correct answer CAP
Joint Commission on Accreditation of Healthcare Organizations. Hospital certifying
agency. - correct answer JCAHO
National Fire Protection Association - correct answer NFPA
Mechanical, Chemical, and Biological - correct answer Name the three classes of
hazards
Time-weighted Average (Amount exposed to over 8 hrs) - correct answer TWA
permissible exposure limits: developed by OSHA to indicate the max airborne
concentration of a contaminant to which an employee may be exposed over the
duration specified by the type of PEL. - correct answer PEL
Short-Term Exposure Limit for exposure during 15 minutes - correct answer STEL
Ceiling Limit - Maximum allowable exposure limit. - correct answer C
Threshold Value Limit - Exposure over an 8 hour work day or 40 hour work week. -
correct answer TVL
Immediately Deadly or Lethal - The level/dose to cause death. - correct answer IDHL
,OSHA defined limit at which something must be done. - correct answer Action Level
TWA: 100ppm
STEL: 150ppm - correct answer TWA & STEL for Xylene
TWA: 0.75ppm
STEL: 2ppm over 15 minutes - correct answer TWA & STEL for Formaldehyde
A - Ash (Wood, paper, or textile)
B - Boiling (Flammable liquid or gas)
C - Current (Electrical)
D - Combustible & Reactive Elements - correct answer Define the classes of fires
Minimum temperature at which a liquid gives off enough vapors to form an ignitable
mixture with air near the liquid's surface. - correct answer Flash Point
Corrects for 2 colors - correct answer Achromic
Corrects for 3 colors - correct answer Apochromic
Object Holder
Blade Holder
Advancement Mechanism - correct answer Identify the 3 main microtome parts
Formed by the face of the block and the inner bevel angle of the knife. 3 to 8 - correct
answer Clearance Angle
5-10 degrees below the melting point of the paraffin used. - correct answer What is
the ideal temperature of a floatation bath.
2-3um - correct answer What is the ideal section thickness for renal biopsies.
3um - correct answer What is the ideal section thickness for bone marrow
4-6um - correct answer What is the ideal section thickness for routine
8-10um - correct answer What is the ideal section thickness for amyloid
8-10um - correct answer What is the ideal section thickness for nerve tissue
Unparalleled block
Uneven chilling or tissue hardening
Chip in the blade - correct answer Causes of a crooked ribbon.
Reposition the block
Chill evenly
,Stir paraffin and re-embed
Move or replace blade - correct answer Solutions for a crooked ribbon.
Aggressive facing
Excessive dehydration
Air bubbles - correct answer Causes of moth holes (holes in section)
More gentle facing
Check processing schedule
Continue cutting if block allows - correct answer Solution for moth holes (holes in
section)
Dull blade
Paraffin too hard or sticky
too much blade tilt
Room temperature too high or low - correct answer Causes of a ribbon not forming
Move or replace blade
Use a paraffin with more plastic
Use a softer paraffin
Decrease blade tilt
Adjust room temperature - correct answer Solution for a ribbon not forming
Dull blade
Too little blade tilt
Warm room
Paraffin too soft - correct answer Cause of section lifting
Move or replace blade
Increase blade tilt
Adjust room temperature
Use harder paraffin - correct answer Solution for section lifting
Hard tissue
Warm microtome
Loose block/blade
Too much blade tilt - correct answer Cause of wash boarding (venetian blinds)
Soak block
Tighten parts
Decrease blade tilt - correct answer Solution for wash boarding (venetian blinds)
Over dehydration
Dull blade
Too much blade tilt
Cutting too rapidly - correct answer Define cause of microvibrations (microchatter)
, Check processing schedule
Replace/Move blade
Decrease blade tilt
Cut slower - correct answer Solution for microvibrations (microchatter)
Too little blade tilt
Loose or worn microtome parts
Hard tissue - correct answer Cause of thick & thin sections
Increase blade tilt
Tighten parts
Soak block - correct answer Solution for thick & thin sections
Dull blade
Dirty blade
Too little blade tilt
Cutting to fast
Room temperature too warm - correct answer Cause of compressed (wrinkled)
sections
Move/replace blade
Clean blade
Increase blade tilt
Cut slower
Cool block or adjust room temperature - correct answer Solutions for compressed
(wrinkled) sections
Knick on blade
Hard particle in block - correct answer Cause of lengthwise scratches on section
Avoid staples and sutures
Surface decal
Soak block
Move/replace blade - correct answer Solution for lengthwise scratches on section
Static electricity - correct answer Cause of flying sections
Breathe on sections
Ground microtome to a water pipe - correct answer Solution for flying sections
Material too hard - correct answer Cause of scratching noise when cutting
Soak block
Surface decal block - correct answer Solution for scratching noise when cutting
100% solved 2023
To detect ferric iron in tissues. Diagnoses idiopathic hemochromatosis - correct answer
What is the purpose of the Prussian Blue stain?
Occupational Safety and Health Administration, a government agency in the
Department of Labor to maintain a safe and healthy work environment - correct answer
OSHA
Environmental Protection Agency
An independent federal agency established to coordinate programs aimed at reducing
pollution and protecting the environment - correct answer EPA
Federal agency that recommends ways to prevent work-related injury. Part of the CDC
and works with OSHA. - correct answer NIOSH
Center for Disease Control - correct answer CDC
College for American Pathologist. Lab accrediting agency. - correct answer CAP
Joint Commission on Accreditation of Healthcare Organizations. Hospital certifying
agency. - correct answer JCAHO
National Fire Protection Association - correct answer NFPA
Mechanical, Chemical, and Biological - correct answer Name the three classes of
hazards
Time-weighted Average (Amount exposed to over 8 hrs) - correct answer TWA
permissible exposure limits: developed by OSHA to indicate the max airborne
concentration of a contaminant to which an employee may be exposed over the
duration specified by the type of PEL. - correct answer PEL
Short-Term Exposure Limit for exposure during 15 minutes - correct answer STEL
Ceiling Limit - Maximum allowable exposure limit. - correct answer C
Threshold Value Limit - Exposure over an 8 hour work day or 40 hour work week. -
correct answer TVL
Immediately Deadly or Lethal - The level/dose to cause death. - correct answer IDHL
,OSHA defined limit at which something must be done. - correct answer Action Level
TWA: 100ppm
STEL: 150ppm - correct answer TWA & STEL for Xylene
TWA: 0.75ppm
STEL: 2ppm over 15 minutes - correct answer TWA & STEL for Formaldehyde
A - Ash (Wood, paper, or textile)
B - Boiling (Flammable liquid or gas)
C - Current (Electrical)
D - Combustible & Reactive Elements - correct answer Define the classes of fires
Minimum temperature at which a liquid gives off enough vapors to form an ignitable
mixture with air near the liquid's surface. - correct answer Flash Point
Corrects for 2 colors - correct answer Achromic
Corrects for 3 colors - correct answer Apochromic
Object Holder
Blade Holder
Advancement Mechanism - correct answer Identify the 3 main microtome parts
Formed by the face of the block and the inner bevel angle of the knife. 3 to 8 - correct
answer Clearance Angle
5-10 degrees below the melting point of the paraffin used. - correct answer What is
the ideal temperature of a floatation bath.
2-3um - correct answer What is the ideal section thickness for renal biopsies.
3um - correct answer What is the ideal section thickness for bone marrow
4-6um - correct answer What is the ideal section thickness for routine
8-10um - correct answer What is the ideal section thickness for amyloid
8-10um - correct answer What is the ideal section thickness for nerve tissue
Unparalleled block
Uneven chilling or tissue hardening
Chip in the blade - correct answer Causes of a crooked ribbon.
Reposition the block
Chill evenly
,Stir paraffin and re-embed
Move or replace blade - correct answer Solutions for a crooked ribbon.
Aggressive facing
Excessive dehydration
Air bubbles - correct answer Causes of moth holes (holes in section)
More gentle facing
Check processing schedule
Continue cutting if block allows - correct answer Solution for moth holes (holes in
section)
Dull blade
Paraffin too hard or sticky
too much blade tilt
Room temperature too high or low - correct answer Causes of a ribbon not forming
Move or replace blade
Use a paraffin with more plastic
Use a softer paraffin
Decrease blade tilt
Adjust room temperature - correct answer Solution for a ribbon not forming
Dull blade
Too little blade tilt
Warm room
Paraffin too soft - correct answer Cause of section lifting
Move or replace blade
Increase blade tilt
Adjust room temperature
Use harder paraffin - correct answer Solution for section lifting
Hard tissue
Warm microtome
Loose block/blade
Too much blade tilt - correct answer Cause of wash boarding (venetian blinds)
Soak block
Tighten parts
Decrease blade tilt - correct answer Solution for wash boarding (venetian blinds)
Over dehydration
Dull blade
Too much blade tilt
Cutting too rapidly - correct answer Define cause of microvibrations (microchatter)
, Check processing schedule
Replace/Move blade
Decrease blade tilt
Cut slower - correct answer Solution for microvibrations (microchatter)
Too little blade tilt
Loose or worn microtome parts
Hard tissue - correct answer Cause of thick & thin sections
Increase blade tilt
Tighten parts
Soak block - correct answer Solution for thick & thin sections
Dull blade
Dirty blade
Too little blade tilt
Cutting to fast
Room temperature too warm - correct answer Cause of compressed (wrinkled)
sections
Move/replace blade
Clean blade
Increase blade tilt
Cut slower
Cool block or adjust room temperature - correct answer Solutions for compressed
(wrinkled) sections
Knick on blade
Hard particle in block - correct answer Cause of lengthwise scratches on section
Avoid staples and sutures
Surface decal
Soak block
Move/replace blade - correct answer Solution for lengthwise scratches on section
Static electricity - correct answer Cause of flying sections
Breathe on sections
Ground microtome to a water pipe - correct answer Solution for flying sections
Material too hard - correct answer Cause of scratching noise when cutting
Soak block
Surface decal block - correct answer Solution for scratching noise when cutting
