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USMLE Step 1 Microbiology Study Guide | Verified | Latest Update 2024

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Bugs that don't G stain well? - These Rascals May Microscopically Lack Color - Treponema, Rickettsia, Mycobacteria, Mycoplasma, Legionella, Chlamydia. Bugs visualized by Giemsa stain? - Borrelia, Plasmodium, trypanosomes, Chlamydia. Bug visualized by PAS stain? - Tropheryma whippelii. Organisms identified by Siehl-Neelsen (carbol fuchsin) stain? - Acid-fast organisms (Mycobacterium > Nocardia). Bug visualized by India ink? - Cryptococcus. Bugs visualized by silver stain? - Pneumocystis, Legionella, (H. pylori?). Culture requirement for H flu? - Chocolate agar w/ factors V and X. Culture requirement for N. gonorrhea? - Thayer-Martin media (VPNT - vancomycin for G+, Polymyxin for G-, Nystatin for fungi; TMP-SMX for Proteus). Culture requirement for B. pertussis? - Bordet-Gengou potato agar ("Bordet") Culture requirement for C. diphtheria? - Tellurite agar, Loffler's media.

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