Histotechnology: Special Stains, etc. questions well answered graded A+

Histotechnology: Special Stains, etc This is a stain for acid mucop olysaccharides and sialomucins. - correct answers Alcian blue This stains reacts with cations and is used to find calcium in tissue. - correct answers Alizaran Red S This stains uses ferric ions to demonstrate mucopolysaccharides and glycoproteins. Must be done at a low pH. - correct answers Colloidal iron In this stain, tissue is first treated with alkali to open up binding sites through the release of hydrogen bonds. - correct answers Congo Red This stain,combined with HCl and paraldehyde, stains elastic fibers and beta cells a deep purple. - correct answers Gomori's Aldehyde Fuschin The first step of this silver stain is the oxidation of hexose sugars into aldehydes by potassium permanganate. - correct answers Gomori's Stain for Reticulin Fibers In this stain, a plasma stain and a CT fibers stain are combined with phosphotungstic acid and acetic acid. Demonstrates collagen and muscle. - correct answers Gomori's Trichrome This stain combines a PAS stain, Weigert's hematoxylin, and a selective collagen stain combining picric acid and methyl blue. - correct answers Lillie's Allochrome This stain uses tungsten in phosphotungstic acid to bind any available hematein, giving a blue lake. - correct answers Mallory's PTAH This is a sequence procedure combining Biebrich scarlet, phosphotungstic or phosphomolybdic acid, and aniline blue. - correct answers Masson's Trichrome In this mucin stain, aluminum is believed to form a chelation complex with carmine. - correct answers Mayer's Mucicarmine This sequence procedures involves alcian blue (converted to monastral fast blue), hematoxylin or resorcin fuschin, Crocein scarlet-aced fuschin, and safran. - correct answers Movat Pentachrome This silver stain oxidizes carbohydrates in basement membranes to aldehydes using periodic acid. - correct answers PAMS This is a sequential cytological stain. - correct answers PAP stain This detects only loosely bound iron, such as hemosiderin, using potassium ferrocyanide. - correct answers Prussian Blue This stain uses ferric chloride and potassium ferricyanide to detect any reducing agents, then is stained in Mayer's Mucicarmine and metanil yellow. - correct answers Schmorl's When sections containing toxic ferrous ions are treated with potassium ferricyanide, this stain is observed. - correct answers Turnbull Blue This regressive procedure overstrains tissue with hematoxylin-ferric chloride-iodine solution to detect elastic fibers. - correct answers Verhoeff-Van Gieson In this stain, certain tissue elements are oxidized to aldehydes by periodic acid. The aldehyde produced then binds the dye. - correct answers PAS reaction This pigment reduces silver. - correct answers Formalin pigment Argentaffin granules are best preserved by what fixative? - correct answers 10% NBF This endogenous pigment can be demonstrated by Oil Red O or Sudan Black B. - correct answers Lipofuschin In an H&E, melanin can be mistaken for what substance? - correct answers Hemosiderin Turnbull Blue uses potassium ___ for iron. - correct answers Ferricyanide Prussian Blue uses potassium ___ for iron. - correct answers Ferrocyanide Either of these stains can be used to differentiate hemosiderin and melanin - correct answers Prussian Blue and Fontana-Masson Alizarin Red S demonstrates what mineral? - correct answers Calcium Nuclei in a PAP stain should be ___ (color). - correct answers Red-purple If nuclei in a PAP stain are brown, this could be due to _____ of the _____ stain. - correct answers Overoxidation; hematoxylin 2.5% Alcian blue stain uses what percentage of acetic acid solution? - correct answers 3% Alcian blue stain for acid mucopolysaccharides uses what percentage of acetic acid solution? - correct answers 1% Colloidal Iron stain is used to detect what? - correct answers Acid mucopolysaccharides This stain for acid mucopolysaccharides will yield dark blue mucins and red nuclei. (Hint: copper-based dye) - correct answers Alcian Blue This stain for mucopolysaccharides and glycoproteins will yield deep blue mucins and pink-red nuclei. (Hint: an ion is absorbed by the mucosubstances.) - correct answers Colloidal Iron