Histotechnology- Microtomy questions well answered graded A+ 2023/2024

Histotechnology- Microtomy1. If there is venetian blind pattern in the section, a possible remedy is to: - correct answers Tighten all screws 2. To decrease the effect of static electricity: - correct answers ground microtome or breathe on it 3. To remedy crooked ribbon formation: - correct answers trim block edges parallel 4. If the specimen is mushy-appearing and sectioning is difficult: - correct answers a. melt block, reprocess & re-embed 6. A scratching noise during sectioning can be caused by: - correct answers tissue too hard 7. If a microtome is old or has been neglected of maintenance, this may be experienced during sectioning: - correct answers sections vary in thickness 8. Contaminates on slides are usually picked up - correct answers from the flotation bath 9. This type of microtome is used for routine paraffin sectioning: - correct answers rotary 10. The edge of a newly sharpened microtome knife should be tested for sharpness with a: - correct answers microscope 11. Which of the following is necessary to ensure a straight ribbon? a. horizontal sides of the block are parallel to each other b. vertical sides of the block are parallel to each other c. horizontal and vertical sides of the block are parallel to each other d. microtome wheel is rotated rapidly - correct answers horizontal sides of the block are parallel to each other 12. By which of the following means does the fluid state of paraffin convert to a solid? a. crystallization b. evaporation c. polymerization d. all of the above - correct answers crystallization 13. Disposable microtome blades should be discarded: - correct answers in a separate 'sharps' box 14. Routine paraffin sections are cut at what micrometer (micron, µm) setting? - correct answers 4-6 µm 15. Sections that vary in thickness or are skipped can be caused by: - correct answers loose screws 16. The temperature of the floatation bath should be how many degrees lower than the melting point of the paraffin used for embedding? - correct answers 5 to 10C 17. One cause of sections being lifted from the knife on the upstroke is that the: - correct answers knife tilt is incorrect 18. Which of the following problems will be seen in paraffin sections that are floated on a water bath that is too cool? a. cracks b. folds c. separations d. stretching - correct answers folds 19. One cause of sections sticking to each other or to parts of the microtome is - correct answers static electricity 20. Which of the following can be used to soften the exposed face of paraffin embedded tissue that is difficult to section, such as a uterus? a. acid b. glycerine c. heat d. xylene - correct answers glycerine 21. The most common reason that a microtome could be considered the cause of poor sections is when the microtome is: - correct answers old or worn out 22. The identification name or number is written on a slide to be H&E stained with: - correct answers lead pencil 23. Which of the following biopsies should be sectioned at 2-3µm? a. bladder b. heart c. kidney d. liver - correct answers kidney 24. Tissue sections with straight lines that appear to run at different angles through the tissue are probably due to: - correct answers quick-freeze spray 25. As the block face is trimmed, the knife digs into the tissue and gouges out a chunk. The most probable cause is: - correct answers tissue is loose in the block 26. Special stains to demonstrate the following tissue elements require tissue cut at 8-10µm? - correct answers amyloid & nerve cells 27. Which of the following is recommended to be cut at 3µm? a. brain b. breast c. liver d. lymph node - correct answers lymph node 28. In relation to the melting point of the wax, the temperature of the drying oven should be set at: - correct answers same as melting point 29. The technique where, after the block is trimmed, the sections are cut and picked up, and then more of the block is trimmed away and more sections are cut and picked up, is called: - correct answers levels 30. Mushy appearing sections can be caused by: - correct answers inadequate processing 31. Which steel knife profile is used for the majority of paraffin microtomy? a. biconcave b. planoconcave c. tool edge d. wedge - correct answers wedge