Histotechnology questions & answers graded A+ already passed

Select the procedure suitable for demonstrating secretory granules in a carcinoid tumor: A. Wilder reticulum B. PAS-aniline blue C. Snook D. Sevier-Munger D. Sevier-Munger The Sevier-Munger technique is an excellent argyrophil procedure for demonstrating carcinoid tumors. It is similar to, but shorter than, the Grimelius. In Bodian's method for nerve fibers and neurofibrils, Protargol impregnates both neurofribrils and connective tissue. Subsequently, connective tissue is rendered colorless by replacement of silver with this metal: A. copper B. gold C. lead D. tin A. copper In Bodian's method, sections are incubated in a solution of Protargol containing metallic copper. The copper replaces silver that has been deposited in connective tissue, resulting in silver impregnation of only nerve fibers and neurofibrils. What is the thickness of most standard glass slides? A. 0.5 mm B. 1.5 mm C. 3.0 mm D. None of the above B. 1.5 mm Which of the following probe types are used to detect a translocation? A. Centromere probes B. Break-apart probes C. Locus-specific probes B. Break-apart probes Break-apart probes target two areas of a specific gene sequence in order to detect a translocation. When a break in the gene sequence occurs, the green and red signal will NOT be close together anymore and will thus appear as separate green and red signals A centromere probe targets the centromeric region of the particular chromosome. These probes allow us to enumerate the number of centromeres and thus chromosomes of a particular kind. A locus-specific probe targets a specific gene sequence of interest. These probes can be used to determine whether a gene is amplified, deleted, or present in a normal copy number. ONLY monoclonal antibodies can be used for immunohistochemical (IHC) techniques for the demonstration of infectious diseases. A. True B. False B. False The histology section of the laboratory had 12,652 labor hours for the year. How many FTEs (full-time equivalents) were used by this section? A. 4. B. 5.70 C. 6.10 D. 9. B. 5.70 To calculate FTEs, or full-time equivalents, divide the total number of hours of all the employees (total paid hours, productive or worked hours, and nonproductive or benefit hours) by 2080, the number of hours a full-time person works in one (1) year. This is calculated by multiplying 40 hrs/week X 52 weeks/yr. = 2080 15. [12,652 total hrs. / 2,080 hr/person = 6.1 FTEs] Fluorescence in situ hybridization (FISH) can be used to qualify patients for chemotherapy. A. True B. False A. True Orth solution is prepared with which of the following constituents? A. mercuric chloride, potassium dichromate, and sodium sulfate B. potassium dichromate, sodium sulfate, and 37-40% formaldehyde C. potassium dichromate, sodium sulfate, and absolute alcohol D.mercuric chloride, sodium sulfate, and 37-40% formaldehyde B. potassium dichromate, sodium sulfate, and 37-40% formaldehyde Helicobacter pylori is a causative bacteria linked to duodenal ulcers, MALT lymphomas, and stomach cancers. A. True B. False A. True Low profile blades (or feathers) require more frequent sharpening, unlike the high profile blades. A. True B. False B. False Low profile and high profile "blades" are disposable and do not require sharpening. Which dye groupe would normally be chosen to stain Nissl bodies: A. quinoneimine B. Azo C. Xanthene D. anthraquinone