Histotechnology--Nuclear & Cytoplasmic Staining fully solved rated A+ already passed 2023/2024

Histotechnology--Nuclear & Cytoplasmic Stainingc. nuclei *hemalum stains (hematoxylin mordanted with either ammonium aluminum sulfate or potassium aluminum sulfate) are used almost exclusively to stain nuclei Harris hematoxylin is used on tissue sections to stain: a. fat b. glycogen c. nuclei d. cytoplasm b. oxidation *can be done naturally (atmospheric oxygen), or with oxidizing agents Ripening of hematoxylin is a process of: a. hydrolysis b. oxidation c. mordanting d. reduction a. hematein *hematoxylin is not a dye The active staining chemical in ripened hematoxylin solutions is: a. hematein b. hematin c. hematoxylin d. hemosiderin c. differentiation in acid-alcohol *regressive means to overstain and then remove excess dye (differentiate) with acid-alcohol The most important step in regressive hematoxylin staining is: a. postmordanting in picric acid b. use of hematoxylin containing glycerin c. differentiation in acid-alcohol d. washing in water after the hematoxylin d. sodium iodate *all choices will oxidize hematoxylin, but sodium iodate is used in Mayer hematoxylin Hematein is formed in Mayer hematoxylin solution by the addition of: a. mercuric oxide b. potassium permanganate c. exposure to air d. sodium iodate b. link tissue constituents more closely to the dye *oxidized hematoxylin has little affinity for tissue but becomes a strong dye with a particular affinity for nuclei when combined with a metallic mordant, linking the tissue constituents more closely to the metal-hematein lake Mordants are used to: a. change the refractive index of the tissue b. link tissue constituents more closely to the dye c. help differentiate stains d. oxidize staining solutions b. poor staining with eosin *because in order to achieve good cytoplasmic staining, the pH of eosin must be acidic, if too much ammonia is carried over into the eosin, cytoplasmic staining will be lacking During H&E staining, if ammonia is incompletely removed by washing, the result may be: a. fading of hematoxylin b. poor staining with eosin c. understained nuclei d. hazy appearance of finished section