HTL Histotechnology Exam 1 questions with 100% correct answers graded A+ 2023/2024

HTL Histotechnology Exam 1The overall magnification of a microscope is achieved by what two values? - correct answers objective lens x eyepiece lens What is the magnification of scanning or low power? - correct answers 2.5x to 4.0x What is the magnification of intermediate power? - correct answers 10x to 20x What is the magnification of high power? - correct answers 40x What is the magnification of high dry? - correct answers 100x What is the magnification of oil immersion? - correct answers 100x What microscope would you use to visualize gout? - correct answers polarizing microscope What microscope would you use with a congo red stain? - correct answers polarizing microscope What microscope would you use for FITC? - correct answers fluorescent microscope What microscope would you use for TEM or SEM? - correct answers electron microscope What instrument would you use to cut sections on paraffin embedded tissue? - correct answers rotary microtome What range of thickness in sections can you achieve cutting on a rotary microtome? - correct answers 1 to 10 microns What is the average thickness of a cut paraffin section using a rotary microtome? - correct answers 3-4 microns List a few challenges that may occur in cutting paraffin blocks? - correct answers tiny specimens, inadequately decalcified tissue, poorly infiltrated tissue, blocks with foreign bodies, dull or poor quality blades, blocks not cooled long enough What is the typical temperature inside a cryostat? - correct answers -20 to -30 degrees Celsius What is the typical temperature maintained in slide drying ovens? - correct answers 65-68 degrees Celsius How long are slides dried for in the drying oven? - correct answers 10-15 minutes What is the temperature range of the different freezers in the lab? - correct answers -10 to -80 degrees Celsius What is the typical temperature range of the refrigerator in the lab? - correct answers 1-5 degrees Celsius What is the term of the instrument used for floating paraffin ribbons? - correct answers flotation bath What temperature should the DI water or tap water be in the flotation baths? - correct answers 10 degrees cooler than the melting point of paraffin What magnification is needed for studying cells and cell structure? - correct answers 400x to 1000x What is the term used with microscopes that means if you have focused on an object using one objective, the microscope will still be coarsely focused when you switch to a different objective. All you need to do is adjust the fine focus a bit. - correct answers parfocal Is the ability of the components of a microscope to measure the angular separation of the points in an object. - correct answers resolution Is the minimum distance between distinguishable objects in an image. - correct answers minimum resolvable distance or resolution What is the resolving power of the naked eye? - correct answers 1 cm to 0.5 cm What is the resolving power of microscopes? - correct answers 0.2 microns Is the artifact produced in microscopy when two different wavelengths of light are passing through an object. - correct answers chromatic refraction (aberration) What part of the microscope corrects for chromatic refraction (aberration)? - correct answers objective _________ objectives correct for two colors (red and blue) - correct answers achromatic _______ correct for 3 colors and are more expensive. - correct answers apochromatic