AMCB 2023 Test Questions with Correct Answers

AMCB 2023 Test Questions with Correct Answers To verify DNA purity we have to: - Answer-Measure absorbance at 260 nm and 280 nm. The addition of proteinase K in the buffer ATL is to: - Answer-Disrupt the cell envelope to lyse cells. The genomic DNA that we extract from cells (of any kind) is polar and sticky. This property is useful for purification purposes. Which of the following surfaces is likely to be used, with good efficiency, to purify DNA. - Answer-A glass rod/bar To quantify DNA we should: - Answer-Measure absorbance at 260 nm. In this course the use of a chimera or chimerical molecule is applied to: - Answer-Genetic recombinant molecules. Select the group describing the type of DNA vectors mentioned in the course: - Answer-Shuttle vectors - Expression plasmids - Reporter plasmids - Suicide plasmids Extraction protocol: Once the bacterial cells are lysed and the cell free extracts are loaded into the column, the plasmids will: - Answer-Be retained on the filter made of glass fibers. The gene SacB encodes for: - Answer-A) The enzyme levan sucrase. B) A toxic enzyme when expressed in E. coli. Select the best definition of a PLASMID: - Answer-A circular genetic structure that replicates independently of the chromosome The use of plasmids is extremely flexible, including the use of viral genes to be inserted and expressed in different kinds of cells. - Answer-True A general characteristic of the plasmids used in biotechnology is a gene conferring antibiotic resistance - Answer-True The bla gene in p15TV-L used in this lab encodes for: - Answer-B) B-lactamase. C) Resistance to ampicillin.