Primavera Biology/checkpoint 13 Questions and Answers 100% Pass

Primavera Biology/checkpoint 13 Questions and Answers 100% Pass Which procedure would help ensure that a new piece of DNA can be inserted inside a bacterial plasmid when the two are mixed together? The fragment and plasmid are both cut with the same restriction enzyme. Which sentences correctly describe restriction enzymes? Select all that apply. They cut DNA strands at specific locations. In bacteria, they function to cut up the DNA of invading viruses A biologist wishes to take a useful gene found in pine trees and introduce it into some bacterial cells. Her experimental procedure is given below. Use a restriction enzyme to cut a gene out of the pine tree DNA. Use a restriction enzyme to cut open a plasmid. Treat the plasmid with DNA ligase. Mix the desired gene and plasmid together. Introduce the plasmid into bacterial cells. Which steps should be switched in the biologist's procedure? 3 and 4 Read the scenario. A biologist inserts a human gene into a plasmid and then inserts the plasmid into some bacterial cells so that the function of the gene can be studied. However, he finds that he cannot identify which bacterial cells contained the new DNA. What should the biologist have done differently? used a plasmid containing the GFP gene A biologist has a mixture of DNA molecules of different sizes, and she needs to determine the exact size of one of them. Which technique should she use? gel electrophoresis A graduate student is running a PCR reaction to study DNA extracted from a lizard specimen from a museum. The reaction does not seem to be working, so he checks his notes to see what he added to the reaction mix. He finds the following list: lizard DNA, DNA polymerase, and DNA nucleotides. What is the error in the setup? He forgot to add primers. A lab technician is using PCR to test a patient's DNA for the presence of a genetic mutation. What exactly will he be looking for? DNA band of a certain size on a gel A scientist is given some frog tissue by a zoo. He is told that the zoo thinks the tissue may be from a rare Chiricahua Leopard Frog. In what order should the following procedures be done to identify the frog? A. Run PCR on the tissue using known Chiricahua Leopard Frog DNA primers. B. Compare the results of the tissue sample and the known sample. C. Run an electrophoresis gel using the sample given by the zoo and a known Chiricahua Leopard Frog DNA sample. A, C, B If a biologist says that she is sequencing a DNA molecule, what is she doing with it? determining the order of nucleotides along the DNA strand How many separate reactions are needed to determine the sequence of DNA using the Sanger method? four Which of the following technologies will make obtaining a copy of your own DNA code quick and affordable? next-generation sequencing