EXAM 1 - Microbiology BIOL 351 – TAMU questions with correct answers

Chapter 1: microbial cell structure and function CORRECT ANSWER Microscopy: compound microscope CORRECT ANSWER •Compound microscope -- Two sets of lenses form the image (Figure 1.15a) •Objective lens and ocular lens Microscopy: magnification CORRECT ANSWER •Total magnification = objective magnification ocular magnification • •Magnification: the ability to make an object larger Microscopy: resolution CORRECT ANSWER •Resolution: the ability to distinguish two adjacent objects as separate and distinct •Resolution is determined by the wavelength of light used and numerical aperture of lens d=0.5λ/numerical aperture •Limit of resolution for light microscope is about 0.2 μm difference btwn magnification and resolution CORRECT ANSWER •Magnification: the ability to make an object larger •Resolution: the ability to distinguish two adjacent objects as separate and distinct •Resolution is determined by the wavelength of light used and numerical aperture of lens d=0.5λ/numerical apert