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Straighterline Microbiology Lab BIO250L Lab 2 Culturing & Aseptic Technique Worksheet 2023

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Pre-Lab Questions 1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What is one experimental way you can test your practices to confirm that you are using proper aseptic technique? By swapping sterile broth in two sterile tubes, you can conduct a test. If you can effectively transport clean broth thoroughly, you can do the same with a pure culture without contamination either one or the space around it. 2. In a laboratory setting, what are three ways you can properly sterilize culturing equipment? a. Flaming b. Autoclaving c. Alcohol 3. For each inoculation tool, give one scenario in which use of that tool would be appropriate.

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