Microscopes
Magnification describes how much bigger an image appears in a microscope compared to the original object.
Microscopes produce linear magnification
The resolution of a microscope is the ability of an optical instrument to see or produce an image that shows fine
detail
Optical microscope-
- cheap
- easy to use
- portable
-can study whole living specimen
- have a limited resolution
- magnification varies from X1500 to X2000
Laser scanning
microscopes-
- use laser light to scan an object point by point
- images displayed are high resolution and show high contrast
Electron microscopes-
- use a beam of fast travelling electrons
- much better resolution than confocal microscopes
- electrons fired from a cathode and focused by magnets
To calculate magnification, you use the
equation
total magnification = power of objective
lens X power of eyepiece lens
Magnification describes how much bigger an image appears in a microscope compared to the original object.
Microscopes produce linear magnification
The resolution of a microscope is the ability of an optical instrument to see or produce an image that shows fine
detail
Optical microscope-
- cheap
- easy to use
- portable
-can study whole living specimen
- have a limited resolution
- magnification varies from X1500 to X2000
Laser scanning
microscopes-
- use laser light to scan an object point by point
- images displayed are high resolution and show high contrast
Electron microscopes-
- use a beam of fast travelling electrons
- much better resolution than confocal microscopes
- electrons fired from a cathode and focused by magnets
To calculate magnification, you use the
equation
total magnification = power of objective
lens X power of eyepiece lens
