AAB MT EXAM TEST HEMATOLOGY LABORATORY METHODS 2023/2024

AAB MT EXAM TEST HEMATOLOGY LABORATORY METHODS 2023/2024 What type of specimen is used for hematology testing? - CORRECT ANSWER-EDTA What type of specimen is used for coagulation testing? - CORRECT ANSWER-3.2% citrated plasma When should a 3.2% citrated plasma be rejected? - CORRECT ANSWER-If the specimen is clotted, hemolyzed or QNS. How is platelet poor plasma obtained from a 3.2 citrated plasma specimen? - CORRECT ANSWER-By centrifuging the specimen. In a coagulation specimen, what is the ratio between anticoagulant and whole blood? - CORRECT ANSWER-1:9 ratio In a coagulation specimen, what is the ratio between whole blood and anticoagulant? - CORRECT ANSWER-9:1 ratio What is the formula for the Red Blood Cell (RBC) Count? - CORRECT ANSWER-(Cells counted X Dilution X 10) / Number of square millimeters counted What is a common source of errors for the RBC Count? - CORRECT ANSWER-Cold agglutinins (due to cold antibodies present). Why is platelet poor plasma essential for coagulation testing? - CORRECT ANSWERPlatelets initiates the coagulation cascade (results in shorten result times). Cold auto-antibodies cases what errors in the CBC test? - CORRECT ANSWER-A falsely decreased HCT and RBC, plus a falsely elevated MCV and MCHC (greater than 37). If a patient has a cold auto-antibody, what can be done to obtain a correct CBC? - CORRECT ANSWER-Incubate specimen at 37 C, reanalyze specimen immediately after incubation, and immediately make the peripheral smear. What is the method for performing a hemoglobin concentration? - CORRECT ANSWER-Cyanmethemoglobin method What is the principle behind the cyanmethemoglobin method? - CORRECT ANSWERThat hemoglobin + ferricyanide yields cyanmethemoglobin The cyanmethemoglobin uses what law, in order to generate results? - CORRECT ANSWER-Beer's law The cyanmethemoglbin cannot be used to determine what type of hemoglobin? - CORRECT ANSWER-Sulfhemoglobin What substances can interfere with Cyanmethemoglobin method? - CORRECT ANSWER-Markedly elevated WBC count (>100X10 to the 9th /L), Lipemia, Abnormal hemoglobins, and Monoclonal globulins (Multiple myeloma and Waldenstrom's). When using the Cyanmethemoglobin method for determining hemoglobin concentration, how can lipemic samples be corrected? - CORRECT ANSWER-Using saline replacement technique (one volume of saline per one volume of plasma removed). Lipemia is caused by what? - CORRECT ANSWER-Increased dietary triglycerides Lipemia should be suspected if? - CORRECT ANSWER-HCT and Hemoglobin results don't match (higher than expected hemoglobin when compared to the HCT). What is another name for hematocrit (HCT)? - CORRECT ANSWER-Packed Cell Volume (PCV) Define the HCT? - CORRECT ANSWER-The % of RBCs in a volume of whole blood. Describe the manual microhematocrit method. - CORRECT ANSWER-Centrifuge two capillary tubes at 10,000 RPMs for 5 minutes and take the average of the two tubes. How is HCT calculated using the automated method? - CORRECT ANSWER-HCT is calcuated using the MCV and RBC What are the RBC indices? - CORRECT ANSWER-* MCV (Mean corpuscular volume) * MCH (Mean corpuscular hemoglobin) * MCHC (Mean corpuscular hemoglobin concentration) * RDW (Red cell distribution width) Degree of anisocytosis How is the MCV calculated? - CORRECT ANSWER-(HCT%/RBC) X 10 How is the MCH calculated? - CORRECT ANSWER-(Hb/RBC) X 10 How is the MCHC calculated? - CORRECT ANSWER-(Hb/HCT) X 100 What is the AAB's reference range for MCV? - CORRECT ANSWER-80-100 fL What is the AAB's reference range for MCH? - CORRECT ANSWER-28-34 pg What is the AAB's reference range for MCHC? - CORRECT ANSWER-32-36 g/dL A peripheral blood smear with a MCV of less than 80 fL will be? - CORRECT ANSWERMicrocytic A peripheral blood smear with a MCV of 80-100 fL will be? - CORRECT ANSWERNormocrytic A peripheral blood smear with a MCV of greater than 100 will be? - CORRECT ANSWER-Macrocrytic A peripheral blood smear with a MCHC of less than 32% will be? - CORRECT ANSWER-Hypochromatic A peripheral blood smear with a MCHC of 32-36% will be? - CORRECT ANSWERNormochromatic A peripheral blood smear with a MCV of greater than 36% will be? - CORRECT ANSWER-Hyperchromatic A peripheral blood smear with a RDW of 12-15 will be? - CORRECT ANSWER-Normal A peripheral blood smear with a RDW of greater than 15 will be? - CORRECT ANSWER-AnisocytosisWhat type of specimen is used for hematology testing? - CORRECT ANSWER-EDTA What type of specimen is used for coagulation testing? - CORRECT ANSWER-3.2% citrated plasma When should a 3.2% citrated plasma be rejected? - CORRECT ANSWER-If the specimen is clotted, hemolyzed or QNS. How is platelet poor plasma obtained from a 3.2 citrated plasma specimen? - CORRECT ANSWER-By centrifuging the specimen. In a coagulation specimen, what is the ratio between anticoagulant and whole blood? - CORRECT ANSWER-1:9 ratio In a coagulation specimen, what is the ratio between whole blood and anticoagulant? - CORRECT ANSWER-9:1 ratio What is the formula for the Red Blood Cell (RBC) Count? - CORRECT ANSWER-(Cells counted X Dilution X 10) / Number of square millimeters counted What is a common source of errors for the RBC Count? - CORRECT ANSWER-Cold agglutinins (due to cold antibodies present). Why is platelet poor plasma essential for coagulation testing? - CORRECT ANSWERPlatelets initiates the coagulation cascade (results in shorten result times). Cold auto-antibodies cases what errors in the CBC test? - CORRECT ANSWER-A falsely decreased HCT and RBC, plus a falsely elevated MCV and MCHC (greater than 37). If a patient has a cold auto-antibody, what can be done to obtain a correct CBC? - CORRECT ANSWER-Incubate specimen at 37 C, reanalyze specimen immediately after incubation, and immediately make the peripheral smear. What is the method for performing a hemoglobin concentration? - CORRECT ANSWER-Cyanmethemoglobin method What is the principle behind the cyanmethemoglobin method? - CORRECT ANSWERThat hemoglobin + ferricyanide yields cyanmethemoglobin The cyanmethemoglobin uses what law, in order to generate results? - CORRECT ANSWER-Beer's law The cyanmethemoglbin cannot be used to determine what type of hemoglobin? - CORRECT ANSWER-Sulfhemoglobin What substances can interfere with Cyanmethemoglobin method? - CORRECT ANSWER-Markedly elevated WBC count (>100X10 to the 9th /L), Lipemia, Abnormal hemoglobins, and Monoclonal globulins (Multiple myeloma and Waldenstrom's). When using the Cyanmethemoglobin method for determining hemoglobin concentration, how can lipemic samples be corrected? - CORRECT ANSWER-Using saline replacement technique (one volume of saline per one volume of plasma removed). Lipemia is caused by what? - CORRECT ANSWER-Increased dietary triglycerides Lipemia should be suspected if? - CORRECT ANSWER-HCT and Hemoglobin results don't match (higher than expected hemoglobin when compared to the HCT). What is another name for hematocrit (HCT)? - CORRECT ANSWER-Packed Cell Volume (PCV) Define the HCT? - CORRECT ANSWER-The % of RBCs in a volume of whole blood. Describe the manual microhematocrit method. - CORRECT ANSWER-Centrifuge two capillary tubes at 10,000 RPMs for 5 minutes and take the average of the two tubes.