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Summary Cloning and Biotechnology Revision Notes

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NEW OCR Biology A Revision Notes (starting teaching from 2015). Part of Module 6 - Genetics and Ecosystems

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Cloning and Biotechnology Revision Notes
Natural Clones

Natural Cloning
-clones are genetically identical copies of either a cell or a whole organism
-cells undergo mitosis and form 2 genetically identical copies of DNA which are separated into 2 identical nuclei to form 2 identical cells

Asexual Reproduction
-any organism that reproduces asexually will produce clones of its self
-single-cell yeast cell reproduce by budding and bacteria reproduce by binary fission will produce clones
-plants also reproduce a sexually to produce clones

Advantages and Disadvantages of Natural Cloning
Advantages Disadvantages
 Conditions for growth suitable for parent then they are suitable  Offspring may become overcrowded
for the offspring  No genetic diversity (unless mutations occur)
 Rapid – population increases rapidly (exponentially)  Population will show little/no variation
 Reproduction can be carried out if there is only 1 parent  Changes in environment may affect whole population

Cloning in Plants

Natural Cloning in Plants
-natural cloning in plants involves a process called vegetative propagation
-vegetative propagation = process of reproduction vegetative parts of the plant rather than specialised reproductive structure
-cell differentiation is not as complete in plants as in animals so the cells can divide into specialised cells or clones of themselves

How Cloning Occurs Naturally

Bulbs
-often bulbs contain more than one apical bud which will each grow into a new plant
-e.g. onions and daffodils

Runners
-a lateral stem grows away from the parent plant over ground and roots develop where the runner touches the ground
-a new plant will develop and the runner will eventually wither away leaving the plant on its own
-e.g. strawberries

Rhizomes
-rhizome is a specialised horizontal stem running underground, often swollen and stored with food
-bunds develop and form new vertical shoots which become independent plants
-e.g. marram grass

Stem Tubers
-tip of an underground stem becomes swollen with stored food to form a tuber
-buds on the tuber develop to produce new shoots
e.g. potatoes

Using Natural Clones in Horticulture
-splitting bulbs, removing young plants from runners and cutting up rhizomes all increase plant numbers cheaply
-also possible to take cutting of many plants and rooting hormone is applied to encourage growth of new roots
-many of the world’s most important food corps are propagated by cloning
 bananas, sugar cane, sweet potatoes from propagated of cutting rhizomes
 coffee and tea bushes propagated from stem cuttings

Producing a Cutting
1) Use a non-flowering stem and make an oblique cut in the stem
2) Use hormone rooting powder and reduce leaves to 2-4 – so that growth is quicker
3) Keep cutting well-watered – ensure that plant is hydrated as water is lost through transpiration
4) Cover the cutting with a plastic bag for few days – prevent disease

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I am medical student aiming that got A*,A*, A at A Level in Physical Education, Biology and Chemistry respectively.. I have sat the new style of linear exams (from 2015 onwards). Please see the notes i have produced as they are what i used to achieve my grades.

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