Clinical Chemistry (Analytical Methods) Questions With Complete Solutions

Wavelength correct answer: Distance between 2 successive peaks (nm) Lower Frequency = Longer wavelength (RED) Higher frequency = Shorter wavelength (VIOLET) Spectrophotometric measurement correct answer: measures light intensity in a narrower wavelength Photometric measurement correct answer: measures light intensity without consideration of wavelength multiple wavelength (uses filter only) LASER correct answer: Light source for Spectrophotometry Visible region correct answer: Tungsten light bulb Mercury arc UV correct answer: Deuterium lamp Mercury Arc Xenon lamp Hydrogen lamp IR correct answer: Merst glower Globar (Silicon carbide) Stray light correct answer: Wavelength outside the band Most common cause of loss of linearity Diffraction gratings correct answer: Most commonly used monochromator Cutting grooves Prisms correct answer: Rotatable monochromator Nickel sulfate correct answer: Prevents stray light Cutoff filter correct answer: Anti-stray light Bandpass correct answer: 1/2 peak transmittance Alumina silica glass cuvet correct answer: Most commonly used cuvet Quartz/plastic cuvet correct answer: UV Borosilicate glass cuvet correct answer: Strong bases Photodetector correct answer: Converts transmitted light into photoelectric energy Barrier layer cell/ photocell/ photovoltaic cell correct answer: Simplest detector