Clinical Chemistry: Instrumentation Questions With Complete Solutions

Spectrophotometry correct answer: Measures the array of lights or radiant energy absorbed or transmitted Beer-Lambert's Law correct answer: Concentration of a substance is directly proportional to the amount of light absorbed, or the logarithm of transmitted light Exciter lamp correct answer: Spectrophotometry: serves as a source of light energy Entrance slit correct answer: Spectrophotometry: controls the amount of light that enters the monochromator Monochromator correct answer: Spectrophotometry: produces light of specific wavelength from the exciter lamp Prisms correct answer: Monochromator: wedge-shaped glass, quartz, of sodium chloride that disperses light to different angles of refraction due to different wavelength Diffractor gratings correct answer: Monochromator: refract (grooves) and diffract (slits) light into different spectra; in an aluminum surface of a flat piece crown glass Interference filters correct answer: Monochromator: composed of semitransparent silver films on both sides of a dielectric (MgF2); filter and allow transmission of 40%-60% of incident light with a bandpass or bandwidth between 10-20 nm Cuvette or analytical cell correct answer: Spectrophotometry: holds the sample to be analyzed Soft glass correct answer: Cuvette: for acidic solution Borosilicate correct answer: Cuvette: for alkaline solution Quartz or plastics correct answer: Cuvettes: routine Photodetector correct answer: Spectrophotometry: detects and collects the light energy transmitted and converts it into a current to produce a readable output