CPPS 325 - Cancer Pt 3 Cancer Stem
Cells Exam Latest Questions And
Verified Answers
CSC - Answer Cancer Stem Cell
Cancer Stem Cell Concept - Answer Proposes the existence of a small population of
cells that have enhanced capacity to seed a tumour
What do those who favour existence of CSCs believe? (2) - Answer - These cells
contribute to metastasis and disease recurrence
- These cells confer chemotherapy
What are the 4 experiments that were used to demonstrate CSCs - Answer 1A: Limited
dilution and transplantation (2x10^5 cells are tumorgenic)
1B: Limited dilution and transplantation (Identifying unique cancerous markers)
2A: Lineage tracing of Epidermal Cancer stem cells
2B: Lineage tracing of Intestinal Cancer cells
1A: Limited dilution and transplantation (2x10^5 cells are tumorgenic) - Answer
Demonstrate which cancer cells can seed a tumour
1) Obtain Normal cancer cells (Acute myeloid leukemia (AML) cells)
2) Create 3 dilutions
- 2x10^4
- 2x10^5
- 2x10^6
3) Identify which dilutions create tumour
- 2x10^4 did NOT
- 2x10^5 and 2x10^6 both formed tumours
4) Use FACS to sort out CSC (by using HSC markers)
- The CD34+/CD38- cells are tumorigenic (CSC)
HSC Markers - Answer - CD34+
- CD38-
, What is the minimum dilution that has tumorigenic cells - Answer Every 1 in 2x10^5
cells are tumorigenic
1B: Limited dilution and transplantation (Identifying unique cancerous markers) -
Answer Collect 2 groups of breast cancer cells and inject them into mouse skin
1) CD44+/CD24-
- Induced tumours
2) CD44+/CD24+
- No tumours induced
Tumour bulk - Answer the mean tumor size of target lesions at baseline
2A: Lineage tracing of Epidermal Cancer stem cells process - Answer 1) Obtain
epidermal cancer stem cells (skin cancer)
- Use K14+ to identify them from papilloma
2) Tag their Reporter gene with YFP
3) Inject the cancer cells into a model animal to make a new papilla
4) TAM Injection (induce the cre-flox system to express the marked reporter)
What Epidermal Stem cell Marker is used to extract epidermal cancer stem cells -
Answer K14+
2A: Lineage tracing of Epidermal Cancer stem cells Results - Answer 6 days post TAM
injection
- There are green fluorescing epidermal cancer stem cells in the dermal papilloma
region
14 Days post TAM injection
- The YFP grows out into the tumour bulk as it is generated
- The movement and growth of the green show that new cells were generated from the
original ones
Normal Stem Cells in the Epidermal Cancer stem cells experiment - Answer Normal
stem cells also express YFP, so they will appear on the imaging, but they do not
contribute to the papilloma
Visualizing the doubling time of the Epidermal Cancer stem cells - Answer Use EdU and
BrdU
- They mimic pyrimidine bases and incorporate themselves into dividing cells
- They are marked for visualization
Cells Exam Latest Questions And
Verified Answers
CSC - Answer Cancer Stem Cell
Cancer Stem Cell Concept - Answer Proposes the existence of a small population of
cells that have enhanced capacity to seed a tumour
What do those who favour existence of CSCs believe? (2) - Answer - These cells
contribute to metastasis and disease recurrence
- These cells confer chemotherapy
What are the 4 experiments that were used to demonstrate CSCs - Answer 1A: Limited
dilution and transplantation (2x10^5 cells are tumorgenic)
1B: Limited dilution and transplantation (Identifying unique cancerous markers)
2A: Lineage tracing of Epidermal Cancer stem cells
2B: Lineage tracing of Intestinal Cancer cells
1A: Limited dilution and transplantation (2x10^5 cells are tumorgenic) - Answer
Demonstrate which cancer cells can seed a tumour
1) Obtain Normal cancer cells (Acute myeloid leukemia (AML) cells)
2) Create 3 dilutions
- 2x10^4
- 2x10^5
- 2x10^6
3) Identify which dilutions create tumour
- 2x10^4 did NOT
- 2x10^5 and 2x10^6 both formed tumours
4) Use FACS to sort out CSC (by using HSC markers)
- The CD34+/CD38- cells are tumorigenic (CSC)
HSC Markers - Answer - CD34+
- CD38-
, What is the minimum dilution that has tumorigenic cells - Answer Every 1 in 2x10^5
cells are tumorigenic
1B: Limited dilution and transplantation (Identifying unique cancerous markers) -
Answer Collect 2 groups of breast cancer cells and inject them into mouse skin
1) CD44+/CD24-
- Induced tumours
2) CD44+/CD24+
- No tumours induced
Tumour bulk - Answer the mean tumor size of target lesions at baseline
2A: Lineage tracing of Epidermal Cancer stem cells process - Answer 1) Obtain
epidermal cancer stem cells (skin cancer)
- Use K14+ to identify them from papilloma
2) Tag their Reporter gene with YFP
3) Inject the cancer cells into a model animal to make a new papilla
4) TAM Injection (induce the cre-flox system to express the marked reporter)
What Epidermal Stem cell Marker is used to extract epidermal cancer stem cells -
Answer K14+
2A: Lineage tracing of Epidermal Cancer stem cells Results - Answer 6 days post TAM
injection
- There are green fluorescing epidermal cancer stem cells in the dermal papilloma
region
14 Days post TAM injection
- The YFP grows out into the tumour bulk as it is generated
- The movement and growth of the green show that new cells were generated from the
original ones
Normal Stem Cells in the Epidermal Cancer stem cells experiment - Answer Normal
stem cells also express YFP, so they will appear on the imaging, but they do not
contribute to the papilloma
Visualizing the doubling time of the Epidermal Cancer stem cells - Answer Use EdU and
BrdU
- They mimic pyrimidine bases and incorporate themselves into dividing cells
- They are marked for visualization
