SBB Exam 2023Questions with correct Answers

Identifying information on the donor form should include: - Answer- Date and time of donation Name Address Telephone # Gender Age or DOB A record of reasons for prior deferrals, if any Donors must be given educational material regarding: - Answer- Infection diseases transmitted by blood transfusion Signs and symptoms of AIDS Risks of procedure Circumstances in which infection disease testing is not performed should be given chance to ask questions Donor qualifications: pay particular attention to the following: - Answer- Donation interval vitals HGB/HCT receipt of blood or other human tissue Immunizations travel infectious diseases Leukapheresis donors may be given what drugs to enhance collections? - Answer- Hydroxyethyl starch Corticosteroids Growth factors donor tubes from each donation are tested for what? - Answer- ABO/RH/ABSC HBsAg anti-HBc Anti-HCV HCV RNA Anti-HIV-1/2 HIV-1 RNA Anti-HTLV-I/II Syphilis ALT is not required by FDA or AABB Plasma manufacturers can use donors positive for what? - Answer- Anti-HBc Anti-HTLV-I/II Cytapheresis and Auto donors must be tested for ID how often - Answer- Every 30 days reactive ID results must be... - Answer- tested in duplicate for confirmation Neutralization: HBsAg and HIV-1 Ag Western Blot - Anti-HIV RIBA - Anti-HCV Anti-HTLV confirmed by repeating the test using another manufacturers EIA test HTLV testing and deferral - Answer- Two+ positives: 2 donations by same manufacturer = indefinite deferral 1+ by one manuf. and 1+ by another manuf = . 1+ by one manuf. and negative by another manuf. = 8 week deferral What does Dextrose in additive do for cells? - Answer- supports ATP generation What does Adenine in additive do for cells? - Answer- provides substrate for ATP synthesis What does sodium biphosphate in additive do for cells? - Answer- controls pH What does citrate in additive do for cells? - Answer- prevents coagulation What is the storage time if additive used is CPD, CP2D? - Answer- 21 days What is the storage time if additive used is CPDA-1? - Answer- 35 days What is the storage time if additive used is AS-1 (Adsol)? - Answer- 42 days What is the storage time if additive used is AS-3 (nutricel)? - Answer- 42 days What is the storage time if additive used is AS-5 (Optisol)? - Answer- 42 days Whole Blood storage temp and description - Answer- 1-6C contains all blood elements plus the anticoagulant-preservative commonly used as a source for component production RBC storage and description - Answer- 1-6C Whole blood with plasma removed RBCs without additives must have final hct <80% Frozen RBC storage and description - Answer- -65C or colder frozen within 6 days if no additive solution RBC w/additive must be frozen before expiration must be put in freezer within 4 hours of removed from 4C Rejuvenated RBC description - Answer- rejuvenation should restore 2,3-DPG to 75% of normal level and ATP above normal levels. If done before 14 days may cause high 2,3-DPG which impairs O2 up-take. AS-3 and AS-5 cannot be rejuvenated Deglyced RBCs storage and description - Answer- 37C warmer or waterbath (20-25 minutes) washed with solutions of decreasing osmolarity to avoid hemolysis. must remove cryoprotectant agents, minimial free hgb, and result in recovery of at least 80% RBC volume. open system:1-6C with 24 hr expiration. closed system: 14 days or as FDA approved What should the osmolality of a deglyced unit be? - Answer- <500. Osmolality is a fxn of free glycerol therefore an increase is a result of improper deglycing What should free hgb in last wash be from decglyed unit? - Answer- <200 Washed RBCs storage - Answer- 1-6C washed with sterile normal saline to remove most of plasma 24 hour expiration FFP storage and description - Answer- prepared from WB or collected using apheresis. frozen at -18C or below contains max levels of labile or nonlabile coag factors Thaw between 30-37C Thawed plasma - Answer- FFP thawed but not transfused within 24 hrs can be stored 1-6C for 5 days reduction in Factors V and VII Liquid plasma - Answer- plasma separated from WB at any time during storage, up to 5 days after the expiration date of WB. supernatant levels of potassium and ammonia are increased Cryo - Answer- prepared from WB collected into CPDA-1 or CPD precipitates when FFP thawed between 1-6C stored at -18C or colder suspended in less than 15mL plasma contains factor VII and fibrinogen and most of XIII single unit store at 20-24 for 6 hrs pooled store at 20-24 for 4 hours platelets - Answer- prepared from WB that hasn't cooled below 20C separated within 4 hours soft spin (plt rich plasma) followed by a hard spin (platelet concentrate) resuspended in about 45-65mL plasma 20-24C with gentle agitation Granulocytes - Answer- usually harvested in buffy coats collection must yield 1.0x10 to the 10th granulocytes Transfuse ASAP Irradiation - Answer- prevents proliferation of transfused T-Lymphs, the primary cause of graft vs host (GVHD) effects of storage on blood components - Answer- decreases 2,3-DPG levels: this controls release of O2 from RBCs, levels recover after 24 hrs post txn decreases ATP increases potassium Leukocyte reduction must reduce WBC count to what? - Answer- <5x10 to the sixth in at least 95% of units samples and yield >85% RBC recovery What must the minimum mean volume of Apheresis RBCs be? - Answer- 180mL packed RBC volume per unit (>60g hgb) 95% of samples units must have at least 150mL (>50g hgb) What must the minimum mean volume of Apheresis RBCs leukoreduced be? - Answer- <5x10 to the sixth WBC and mean volume of 153mL packed RBC volume per unit (>51g Hgb) 95% of sample units have at least 128mL (>42.5g hgb) How much coag factor VIII and fibrinogen must cryo have? - Answer- 80IU Factor VIII and 150mg fibrinogen pooled units must have same amt multipled by number of units in pool What are Mendel's principles? - Answer- random segregation: distinct genes are randomly inherited one from each parent what is independent assortmant (Mendel's principle)? - Answer- genes inherited independently if carried on different chromosomes. combos of genes are not dependent on other genes (exception: linkage) what is an autosome? - Answer- any chromosome other than the sex chromosomes What is meiosis? - Answer- process by which sex cells are produced which have only one copy of each chromosome (haploid). What is mitosis? - Answer- process where sex cells are duplicated and equally divided between two daughter cells zygote - Answer- fertilized egg gamete - Answer- sex cells....egg and sperm diploid - Answer- normal number of chromosomes in zygote and all body cells (46) haploid - Answer- normal number of chromosomes in all gametes (23) structural gene - Answer- gene that controls the protein production of another gene or genes regulator gene - Answer- gene that controls the rated of production of another gene or genes