PROJECT IMPLEMENTATION
AND REVIEW
The antibiotic susceptibility testing
Unit 6 Assignment C/D
Investigative Project
,Project implementation and ReviewApril 20, 2023
Table of Contents
The antibiotic susceptibility testing.......................................................................................................2
Aim....................................................................................................................................................2
Introduction.......................................................................................................................................2
Hypothesis.........................................................................................................................................3
Variables (Dependent & Independent)..............................................................................................4
Apparatus/ Materials.........................................................................................................................4
Risk assessment.................................................................................................................................5
Method..............................................................................................................................................6
Results...............................................................................................................................................7
T-Test calculation...........................................................................................................................8
T-Test calculation.........................................................................................................................10
Conclusion.......................................................................................................................................11
Evaluation........................................................................................................................................14
Skills learnt from the experiment................................................................................................15
Bibliographies......................................................................................................................................16
1
, Project implementation and ReviewApril 20, 2023
The antibiotic susceptibility testing
Aim
To determine which antibiotics are most effective for treating a bacterial
infection, antibiotic sensitivity tests are performed. There is a likelihood
that an antibiotic will be effective in killing or sufficiently inhibiting the
growth of specific bacteria causing an infection based on its susceptibility.
[1]
The bacterium can only be killed by certain classes of antibiotics.
Different antibiotics can be used to determine whether a bacteria is
susceptible to them. Antibiotic susceptibility testing is used to determine
whether bacteria can grow after being exposed to a variety of antibiotics.
[1]
Introduction
Antimicrobial drug susceptibility tests expose a standardized
concentration of organisms to specific concentrations of antimicrobial
drugs in order to determine a microbe's vulnerability to them.[2] Viruses,
bacteria, and fungi can all be tested for resistance. A drug's performance
can be predicted by a drug of a similar type for some organisms. The
testing of all potentially useful drugs does not always take place.
Zones of inhibition are circular areas around antibiotic spots where
bacteria colony growth is inhibited. Bacteria's susceptibility to antibiotics
can be measured by measuring their zone of inhibition.[9]
Testing for susceptibility is typically conducted in vitro and may not fully
account for a number of factors that influence treatment success at the
site (e.g., pharmacokinetics, pharmacodynamics, host immune status,
host defences specific to the site).[2] The result of a susceptibility test does
not always predict the outcome of a treatment.
There are two bacteria that the susceptibility test will be done on.
The first bacterium is Escherichia coli, in short E.coli, which can be found
in humans, food, and animals' intestines.[3] E. coli is one of the most
diverse groups of bacteria. Despite the fact that most strains of E. coli are
harmless, other strains can cause illness. It has been found that some
types of E. coli can cause diarrhoea, while others can cause pneumonia,
respiratory illness, and urinary tract infection.[3] Normally anaerobic and
Gram-negative bacteria, Escherichia coli (E. coli) is a rod-shaped rod. [4]
Gram-positive and Gram-negative infections are the two main categories
of bacterial infections. Gram stains are used to diagnose the categories
2
AND REVIEW
The antibiotic susceptibility testing
Unit 6 Assignment C/D
Investigative Project
,Project implementation and ReviewApril 20, 2023
Table of Contents
The antibiotic susceptibility testing.......................................................................................................2
Aim....................................................................................................................................................2
Introduction.......................................................................................................................................2
Hypothesis.........................................................................................................................................3
Variables (Dependent & Independent)..............................................................................................4
Apparatus/ Materials.........................................................................................................................4
Risk assessment.................................................................................................................................5
Method..............................................................................................................................................6
Results...............................................................................................................................................7
T-Test calculation...........................................................................................................................8
T-Test calculation.........................................................................................................................10
Conclusion.......................................................................................................................................11
Evaluation........................................................................................................................................14
Skills learnt from the experiment................................................................................................15
Bibliographies......................................................................................................................................16
1
, Project implementation and ReviewApril 20, 2023
The antibiotic susceptibility testing
Aim
To determine which antibiotics are most effective for treating a bacterial
infection, antibiotic sensitivity tests are performed. There is a likelihood
that an antibiotic will be effective in killing or sufficiently inhibiting the
growth of specific bacteria causing an infection based on its susceptibility.
[1]
The bacterium can only be killed by certain classes of antibiotics.
Different antibiotics can be used to determine whether a bacteria is
susceptible to them. Antibiotic susceptibility testing is used to determine
whether bacteria can grow after being exposed to a variety of antibiotics.
[1]
Introduction
Antimicrobial drug susceptibility tests expose a standardized
concentration of organisms to specific concentrations of antimicrobial
drugs in order to determine a microbe's vulnerability to them.[2] Viruses,
bacteria, and fungi can all be tested for resistance. A drug's performance
can be predicted by a drug of a similar type for some organisms. The
testing of all potentially useful drugs does not always take place.
Zones of inhibition are circular areas around antibiotic spots where
bacteria colony growth is inhibited. Bacteria's susceptibility to antibiotics
can be measured by measuring their zone of inhibition.[9]
Testing for susceptibility is typically conducted in vitro and may not fully
account for a number of factors that influence treatment success at the
site (e.g., pharmacokinetics, pharmacodynamics, host immune status,
host defences specific to the site).[2] The result of a susceptibility test does
not always predict the outcome of a treatment.
There are two bacteria that the susceptibility test will be done on.
The first bacterium is Escherichia coli, in short E.coli, which can be found
in humans, food, and animals' intestines.[3] E. coli is one of the most
diverse groups of bacteria. Despite the fact that most strains of E. coli are
harmless, other strains can cause illness. It has been found that some
types of E. coli can cause diarrhoea, while others can cause pneumonia,
respiratory illness, and urinary tract infection.[3] Normally anaerobic and
Gram-negative bacteria, Escherichia coli (E. coli) is a rod-shaped rod. [4]
Gram-positive and Gram-negative infections are the two main categories
of bacterial infections. Gram stains are used to diagnose the categories
2
