Summary CHEME 3320 Recitation 12
o precipitate catalase from an enzyme mixture which includes cholesterol oxidase, our desired product. Catalase has a molecular weight of 250,000, a precipitate density of 1.3 g/cm3 , an average diameter in solution of 10 nm, and an average diffusion coefficient of 4.1 x 10-7 cm2 /sec. It is to be precipitated by a sudden pH change in a 100 liter tank stirred with a 0.1 hp motor. The feed concentration is 0.2 g/liter and sticking coefficient is about 0.05 sec. Assuming that nucleation is fast and flocculation is negligible, and that the solution has properties close to those o
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Cornell University
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CHEME 3320
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