ABO Blood Grouping question with correct answers 2023

ABO Blood Grouping question with correct answers 2023What is the ABO system? - correct answer is the only system is which there is a reciprocal relationship between the antigens on the red cell and the naturally occurring antibodies in the serum What should routine grouping of donor include? - correct answer both cell and serum tests, each serving as check on the other What is the procedure based on? - correct answer based on agglutination of antigen positive red cells in the presence of antibody directed towards the antigen What is the difference of agglutination in forward and reverse type? - correct answer -agglutination of RBCs with a given reagent is a positive test result, which indicates the presence of the corresponding antigen on the RBCs (forward group/type)--ANTIGEN -direct agglutination of A1 or B reagent red cells with the patient serum/plasma indicates the presence of the appropriate ABO antibody (reverse group/type)--ANTIBODY How do you make a 2-5% cell suspension for forward grouping? - correct answer 1. draw blood up to line as shown in pic 2 transfer the blood to a clean and labeled tube (patient last name and first name initial, ID number, "cells", your initials) 3. fill the test tubbe with saline and gently resuspend 4. centrifuge 1 min 5. "dump" saline--end of first wash 6. after decanting, resuspend cells by gently shaking the tube making sure all the cells are in the bottom of the tube--add saline making sure the saline stream is hitting the bottom of the test tube and it makes the "swoosh" sound (you should get foam at the top of the tube) 7. centrifuge for 1 min 8. decant, and repeat one more time for a total of three times 9. after the third dump resuspend--carefully add saline to match the color of the reverse grouping cells **YOU DO NOT NEED A FULL TEST TUBE OF BLOOD --you only need 3 drops of blood to perform the ABO grouping What is the procedure for preparing a patient's sample for reverse typing? - correct answer 1. centrifuge patient sample to separate cells and plasma (2-5 minutes) 2. label test tube with patient last name, first name initial, ID # and your initials and "PLASMA" 3. perform reverse grouping Tube method - correct answer -make sure to leave at least three spaces in between the forward and reverse typing tubes What should you always put in the tubes first? - correct answer antisera; this is the quality control measure to assure that antibody has been added to the test procedure and will help to eliminate false negatives Do you have to perform forward and reverse typing at the same time? - correct answer yes; it is not necessary to do the procedures separately What is the patient's sample collected in for this procedure? can the testing be delayed? what can cause a false result? - correct answer EDTA; specimen should be tested as soon as possible after collection but EDTA and clotted specimens may be stored at 2 to 8C for up to 10 days if there is a delay in testing -storage may result in weaker than normal reactions -bacterial contamination of the specimen may cause false test results What are the three reagents used? - correct answer 1. blood grouping reagent: anti-A, anti-BB, and anti-AB 2. reagent red blood cells: A1 and B cells 3. physiologic saline