MCB 101: LON CAPA 2023 verified

MCB 101: LON CAPA 2023 verifiedStreak Plate vs. Spread Plate For each statement, match it correctly with either Streak Plate or Spread Plate. Dilutes cells by mechanical separation. - correct answer Streak Plate Streak Plate vs. Spread Plate For each statement, match it correctly with either Streak Plate or Spread Plate. Dilutes cells prior to inoculating media. - correct answer Spread Plate Streak Plate vs. Spread Plate For each statement, match it correctly with either Streak Plate or Spread Plate. Goal is to determine original CFU/mL. - correct answer Spread Plate Streak Plate vs. Spread Plate For each statement, match it correctly with either Streak Plate or Spread Plate. Goal is to generate pure culture. - correct answer Streak Plate Increasing which of the following will improve the resolution of the image seen in the microscope? -Numerical aperture of the lens used to view the specimen. -Wavelength of the light used to illuminate the specimen. -Intensity of the light used to illuminate the specimen. -Magnification of the objective used to view the specimen. - correct answer Numerical aperture of the lens used to view the specimen. A student plates 100 µL of a 10E-7 dilution of E. coli culture on a Luria Agar plate. After 24 hour, 73 colonies have grown on the plate. What was the original concentration of the culture in CFU/mL? - correct answer 0 (7.3E9) What Numerical Aperture is required to obtain a resolving power of 0.25 µm if the light source has an average wavelength of 540 nm? - correct answer 1.08 Why is it important to allow a sterilized inoculating loop to cool before attempting to sample liquid culture? - correct answer to avoid creating aerosols Gram negative cells appear what color? - correct answer pink Gram positive cells appear what color? - correct answer purple For a Gram negative cocci, how would the cells appear after the following steps of a Gram stain? Safranin - correct answer pink For a Gram negative cocci, how would the cells appear after the following steps of a Gram stain? Iodine - correct answer purple For a Gram negative cocci, how would the cells appear after the following steps of a Gram stain? Ethanol - correct answer colorless