Lon Capa with correct answers 2023

Lon Capa with correct answers 2023What should you do when you first pick up your inoculating loop? - correct answer Flame sterilize it from stem to loop If you have immersion oil on your slide, which objective can you not use? - correct answer 40x How do you heat fix a slide? - correct answer Pass the back of the slide through the flame of your Bunsen burner 3 times after it has air dried If you held your slide up to light just after the smear had completely air dried, how should the smear appear? - correct answer a light haze Streak Plate vs. Spread Plate - correct answer Streak: - dilutes cells by mechanical separation - goal is to generate pure culture Spread: - dilutes cells before inoculation - goal is to determine original CFU Sterilization vs. Disinfection - correct answer sterilization - kills all microorganisms disinfection - kills many microorganisms but not all How can you improve the resolution of the image seen in the microscope? - correct answer increase numerical aperture decrease wavelength A student plates 100 µL of a 10-7 dilution of E. coli culture on a Luria Agar plate. After 24 hour, 104 colonies have grown on the plate. What was the original concentration of the culture in CFU/mL? - correct answer 10,400,000,000 What Numerical Aperture is required to obtain a resolving power of 0.2 µm if the light source has an average wavelength of 670 nm? - correct answer 0.1493 mordant for: gram stain simple stain spore stain capsule stain - correct answer iodine none heat none Which staining method used in MCB101 cannot be heat-fixed? - correct answer capsule stain what is the correct decolorizer for each type for staining: spore acid fast