BISC 440 Lab 4: Gene Regulation questions and answers,100% correct

BISC 440 Lab 4: Gene Regulation questions and answers,100% correct Observe the screen next to the microscope to identify the cells in this cell culture. What type of cells do you have on this plate? a. stem cells b. muscle cells c. neurons d. blood cells a why are induced pluripotent stem cells (iPS) used instead of embryonic stem cells (ES)? a. iPS have more ethical concerns that have to be considered b. high efficiency in establishing the cell line c. cheaper to implement d. iPS are better to avoid immunological reactions the ES d The diagram above shows a eukaryote cell where the central dogma of molecular biology is represented. This dogma shows the flow of genetic information. The correct terms to fill boxes A, B,C, and D are... a. transcription, pre-mRNA, mRNA, ribosome b. translation, pre-mRNA, mRNA, golgi apparatus c. translation, mRNA, pre-mRNA, mitochondria d. transcription, ribosome, pre-mRNA, mRNA a in eukaryotes a combination of molecules and a region of DNA will increase the gene expression rate. What are the molecules? a. repressors and an enhancer region b. activators and terminator c. transcription factors and promoter d. transcription factors and an enhancer region d iPS can be developed by altering the gene expression in somatic cells using an adequate set of transcription factors. Every cell in our body uses its own set of transcription factors to regulate gene expression. Which set of transcription factors would you use? a. unique transcription factors that can only be found in stem cells b. general transcription factors that can be found in every cell a Transduction is the process whereby foreign DNA is introduced into another cell via a viral vector. In this lab, four transcription factor genes were transduced into fibroblast cells. Which virus did you use today? a. 1 b. 4 c. 2 d. 3 d based on your examination of the morphology of the cell lines, which cell line exhibits stem-like morphology? a. it cannot be determined b. the one that was transduced with the unique transcription factors c. neither of them d. the one that was transduced with the general transcription factors b The gene expression of NANOG is unregulated in iPS (Induced Pluripotent Stem) cells but silenced in differentiated cells. What does this suggest about NANOG? a. is not significant in maintaining pluripotency b. sustains stem cells self-renewal ability c. is expressed at all stages of cell development d. promotes differentiation of cells b why do we need to analyze these samples (RT-PCR results from embryonic stem cells and fibroblast cells) as well? a. we do not need to analyze these samples b. because they will give information about the transcription factors c. because those samples are the experimental controls d. because it's good to have as many samples as possible c Examine the PT-PCR result that shows gene expression of NANOG in the cells. Which cells express NANOG? a. fibroblast cells b. cells that have been transduced with general transcription factors c. all cells d. cells that have been transduced with unique transcription factors d