Triple Sugar Iron Agar (TSI) Question 100% solved

Triple Sugar Iron Agar (TSI) Question 100% solvedWhat is the TSI test used to determine? It is used to determine fermentation and sulfur reduction in Gram negative enteric bacteria What are the 3 sugars used in the TSIA test? Sucrose (present in high concentrations) Lactose (present in high concentrations) Glucose (present in low concentrations) K/A H2S+ K/K H2S- K/A H2S- A/A H2S- Control Sucrose Disaccharide Lactose Disaccharide Glucose Monosaccharide Triple sugar iron agar is used to test for the ability of an organism to: Ferment glucose and/or lactose Produce H2S In TSI agar, if both the slant and the butt (bottom) appear pink after incubation for 48 hours at 37C, then we can say that: Protein catabolism took place Which two ways is hydrogen sulfide produced? When thiosulfate serves as final electron acceptor and is therefore reduced, H2S is produced by-product Metabolization of the amino acid cysteine produces H2S What does TSI media contain to allow for the detection of the colorless hydrogen sulfide? FeSO4 Which of the following is the pH indicator in the TSI agar? Phenol red If the organism does not produce the enzymes to catabolize the other sugars it will switch to protein catabolism. You will see the change in pH in the ___ first. Slant