BIOD171 Microbiology Lab Notebook | Complete Rated A+ | Portage Learning

BIOD171 Microbiology Lab Notebook | Complete Rated A+ | Portage Learning. Need lens, object, and light source. Adjust how large an object becomes. Light Microscope-halogen light bulb. Light pieces (oculars), Arm/Neck, Objectives (4x, 10x, 40x, 100x; Dry vs.Oil), Stage: Clamp Holder, Stage Clips, Focus Knobs (outer and inner), Iris/Diaphragm, Base Power of objective x Power of eyepiece=Total magnification Results: Magnification=objective x eyepiece Lab 3 Notebook Title: SR03 Mounting Techniques Objective: Microscopic examination of bacterial samples through various staining techniques. Identify color and shape of given samples. Procedure: Dry Mount 1. Clean Slide (70% ethanol) 2. Circle area on slide for easy location of specimen (optional) 3. Apply organism to slide: a. If from culture, use sterile loop to spread onto slide b. If from plate, use sterile loop to pick colonies and mix with a drop of distilled water 4. Air dry at room temperature until all moisture has evaporated. Wet Mount 1. Clean Slide (70% ethanol) 2. Circle area on slide with a wax/hydrophobic pen 3. Apply organism to slide: a. If from culture, use sterile loop to spread onto slide b. If from plate, use sterile loop to pick colonies and mix with a drop of distilled water