BIOS 242 Week 2 Lab 1; Microscopy

Lab3:Microscopy-ObservationofCells LearningObjectives: • Usethecompoundlightmicroscopeforobservationofcells. • Identifydifferenttypesofbacteriabasedontheirshapes. • Identifyavarietyofeukaryoticcells. Introduction: Exercise2:ObservationofDifferentTypesofBacteriaMaterials: Compoundmicroscope,immersionoil,lenswipes,preparedslidesofvariousbacteria-Rods,cocciandspiral Method: 1. Obtainthepreservedslideofabacteria. 2. UseAppendixAandfollowtheinstructionsonhowtouseaMicroscope. 3. Observetheslideunder10X,andthen40Xobjectivelenses. 4. Recordanddescribeyourobservationsmadeusing40Xlens. 5. Makesuretonoteasmanydetailsaspossibleaboutthesize,shapeandcolor(stain)ofbacteria. 6. Use100Xoilimmersionlensifneededandrecordyourobservations. Note to Students: Don’t forget to use oil with 100X lens only. After completing the observation, clean the oilimmersionlenswithlenswipestoremoveoil.Becarefultonotcontaminateotherlenses(4X,10X,40X)withoilasthiswillcausedamage tothe otherlenses. 7. Repeatthesteps1-6forslidescontainingbacteriaofdifferentshapesasneeded.Recordyourobservationsinthelabreport. Exercise3:ObservationofEukaryoticCells-ProtozoaandFungiMaterials: CompoundMicroscope,preparedslidesofProtozoaandfungus Method: Protozoa: 1. Obtainthepreservedslideofaprotozoa. 2. UseAppendixAandfollowinstructionsonhowtouseaMicroscope. 3. Observetheslideusing10X,and40Xobjectivelenses. 4. Recordanddescribeyourobservationsmadeusing40XintheLabreport.Makesuretonoteasmanydetailsaspossibleaboutthe size,shapeandcolor(stain)of protozoa. Fungi: 1. Obtainthepreservedslideofamulticellularfungi. 2. UseAppendixAtofollowinstructionsonusingaMicroscope. 3. Observetheslideusing10X,and40Xobjectivelenses. 4. Recordanddescribeyourobservationsmadeusing40XintheLabreport.Makesuretonoteasmanydetailsaspossibleabout thesize, shapeandcolor(stain)of fungus. Lab3Report:Microscopy Exercise1:PartsofMicroscope Table1:Calculatethetotalmagnification LabelthepartsofMicroscope: Questions: Calculatethefinalmagnification: 1. 20xobjectiveand10xeyepiece 2. 50xobjectiveand15xeyepiece Exercise2:ObservedifferentTypesofbacteria Drawtheobservationinsidethecirclesprovided.Labelyourimageappropriately.Writethemagnificationatwhichtheobservationwas made. BacterialSlideof: Questions: 1. Whatthreebacterialshapesdidyouobserve? 2. Howdoesincreasedmagnigicationaffectthefieldofvision? 3. Whyistheoilimmersionobjectivenecessarytodeterminethemorphologyofprokaryotes? 4. Whyisitdesirablethatmicroscopeobjectivesbeparfocal? 5. Whichobjectivefocusesclosesttotheslidewhenitisinfocus? 6. Whichcontrolsonthemicroscopeaffecttheamountoflightreachingtheocularlens? Exercise3:ObservedifferenttypesofEukaryotes-ProtozoaandFungi Drawtheobservationinsidethecirclesprovided.Labelyourimageappropriately.Writethemagnificationatwhichtheobservationwas made. EukaryoticSlideof: EukaryoticSlideof: __ Questions: 1. Didyouneedoilimmersiontoobservetheshapeofeukaryotes?Whyorwhynot? 2. DescribesomeofthevisualdifferencesyouobservedbetweenbacteriaandEukaryoticcells. 3. CLINICALAPPLICATION:Whatistheimportanceofflushingeyewashstationsfrequently?