Biotechnology: Techniques of Molecular Biotechnology questions with correct answers

Recombinant DNA Technology, what are the main concepts? what are the applications? CORRECT ANSWER -Making large quantities of DNA and proteins from non-human sources by combining gene(s) with cloning vector. -Making therapeutic proteins and genes Polymerase Chain Reaction, what are the main concepts? what are the applications? CORRECT ANSWER -Amplifying or making copies of specific DNA segments in vitro -Diagnostics, forensics, paternity testing Blotting Techniques, what are the main concepts? what are the applications? CORRECT ANSWER -Separating nucleic acid and protein samples in a gel and transferring them to a solid support so that they can be detected conveniently *only technique used in this class to be able to isolate protein or DNA or RNA o interest to be able to purify the protein to be able to sequence it -Identify DNA, RNA, and protein molecules Immunoassay, what are the main concepts? what are the applications? CORRECT ANSWER -Detecting proteins (used to detect any protein) using antibodies -Diagnosis, therapeutic drug monitoring (ex. ELISA test kit DNA Microarray, what are the main concepts? what are the applications? CORRECT ANSWER -Detecting a large number of genes simultaneously by probe hybridization technique -Drug target identification, diagnostics Next Generation Sequencing, what are the main concepts? what are the applications? CORRECT ANSWER -High throughput sequencing technique using shotgun & bioinformatics approaches -Targeted therapy, guiding drug therapy