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Zusammenfassung Mikroskopie

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Mikroskopie: Lichtmikroskopie vs. Elektronenmikroskopie - Fluoreszenzmikroskopie - konfokale Laserscanningmikroskopie - STED-Mikroskopie - TEM - REM - Vor- und Nachteile

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n~itnum~onr.ve



LICNTMIKROSKOPIE Elektronenmikroskopie


Übersicht bei grópseren Objekten >
unübersichtlich Kleine PRÜ Parate
> sichtbarkeit Kbender Zeller >
nur tote Zeller
>
farbige Biker > nur graustufen
>
AUHÓSUNG 1000 fach geringer > hohe AUHÓSUMG
> ráumliche Darstellung
v >
Aufwand ,
leitintensitcit
FluorescenteMIKROSKOPIC :
>
Koster
>
Farbstoffe binder an lellbestandteile > farblose

strukturen ( die fluoresrierende Farbstoffe binder) v




sichtbar machen TEM (Transmissionselektronenmikroskop) :




v
>
Elektronenstrahl durchdringt dünnes Prciparat

Konfokak Laserscanningmikroskopie
>
winzige strukturenim Zellinneren sichtbar
> hcihere AUHÓSUNG durch Lochblende , I Laserstrahl

v
>
REM (Rasterelektronenmikroskop)
STED -


MIKROSKOPIE Cstimulated emission depktion) s Elektronenstrahl tastet Oberfliicheab
> AUS Schatten der Hintergrundfluoreszenz >
oberfliichenstrukturen

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Uploaded on
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Number of pages
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Written in
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