Unit 1, M4
, Abstract:
• This information outlines the advantages and disadvantages and the comparisons between both the light and electron microscope. Each type
designed for differences and functions of each unique microscope. The first electron microscope was constructed in 1931 compared to the lig
which is a very recent invention.
Method:
• Although the light microscope is the most commonly used biological instrument, it is often used incorrectly. For the reason that the lens syste
microscope are composed of many lenses; it is called compound. The typical illumination of specimens in which light passes through the spe
to your eye is called bright field microscope. I will now explain how to use and setup a light microscope. Carefully, you should take out the m
closet and place it on the lab bench. One hand should be on the neck of the body and the other underneath. The protective cover should be
the power cord must be plugged in. Next, gently place the slide to be observed on the stage and hold it in place using the metal dips. The su
must be placed directly over the hole in the stage. After placing the slide on the stage, look on the outside and use the coarse focussing knob
as close to the objective as possible without touching it. Now you can look into the eye piece and slowly move the stage away from the objec
as something comes into view, you will need to verify that it is really something on the slide and not just a piece of dust on or inside the micr
this, gently move the slide back and forth whilst looking into the eyepiece. If the image you see moves then it is correct; you are looking at th
in the image moves as you move the slide, then, you are not looking at the slide. Instead you are looking at an impunity.
Discussion:
• Between these two microscopes, there are not many things that these two particular microscopes have in common. Both electron and light m
technical devices which are used to visualize the structures that are two small to see with the naked eye; both types also have relevant area
biology and the materials sciences. However the methods of visualizing the structures is very different.
• The magnification of an electron microscope is greater of that of a light microscope as an electron microscope has a magnification of only 10
Adding to this, an electron microscope has a greater depth of field than a light microscope, however a light microscope is much cheaper than
microscope. It also includes a colour image which is much easier to investigate rather than the black and white image of an electron microsc
microscopes can also view live or dead specimens however electron microscopes can only view the dead ones. Furthermore, a light microsco
portable and can therefore be carried around easily with no hassle, whereas an electron microscope requires special large rooms and expert
Electron microscopes are generally affected by magnetic fields which can cause problems when investigating certain specimens, whereas a l
unaffected by the magnetic fields which can cause problems when investigating certain specimens, whereas a light microscope is unaffected
fields.
Conclusion:
• Overall I believe the electron microscope is better than the light microscope to an extent; in terms of greater resolution and greater magnific
1,000,000x. This is extremely greater than the magnification of a light microscope which is only a poor 1000-1500x compared to the electron
However in terms of price, colour image and simple preparations, the light microscope wins by a mile. This is because it is in colour image, a
prepared by anyone which boosts its simplicity factors. It is also significantly cheaper than electron microscopes which are very expensive. Ta
consideration, I believe both microscopes are excellent for their own particular features and factors.
Bibliography:
• www.slideshare.net/samithsenadeera/comparison-of-light-and-electron-microscopes
, Abstract:
• This information outlines the advantages and disadvantages and the comparisons between both the light and electron microscope. Each type
designed for differences and functions of each unique microscope. The first electron microscope was constructed in 1931 compared to the lig
which is a very recent invention.
Method:
• Although the light microscope is the most commonly used biological instrument, it is often used incorrectly. For the reason that the lens syste
microscope are composed of many lenses; it is called compound. The typical illumination of specimens in which light passes through the spe
to your eye is called bright field microscope. I will now explain how to use and setup a light microscope. Carefully, you should take out the m
closet and place it on the lab bench. One hand should be on the neck of the body and the other underneath. The protective cover should be
the power cord must be plugged in. Next, gently place the slide to be observed on the stage and hold it in place using the metal dips. The su
must be placed directly over the hole in the stage. After placing the slide on the stage, look on the outside and use the coarse focussing knob
as close to the objective as possible without touching it. Now you can look into the eye piece and slowly move the stage away from the objec
as something comes into view, you will need to verify that it is really something on the slide and not just a piece of dust on or inside the micr
this, gently move the slide back and forth whilst looking into the eyepiece. If the image you see moves then it is correct; you are looking at th
in the image moves as you move the slide, then, you are not looking at the slide. Instead you are looking at an impunity.
Discussion:
• Between these two microscopes, there are not many things that these two particular microscopes have in common. Both electron and light m
technical devices which are used to visualize the structures that are two small to see with the naked eye; both types also have relevant area
biology and the materials sciences. However the methods of visualizing the structures is very different.
• The magnification of an electron microscope is greater of that of a light microscope as an electron microscope has a magnification of only 10
Adding to this, an electron microscope has a greater depth of field than a light microscope, however a light microscope is much cheaper than
microscope. It also includes a colour image which is much easier to investigate rather than the black and white image of an electron microsc
microscopes can also view live or dead specimens however electron microscopes can only view the dead ones. Furthermore, a light microsco
portable and can therefore be carried around easily with no hassle, whereas an electron microscope requires special large rooms and expert
Electron microscopes are generally affected by magnetic fields which can cause problems when investigating certain specimens, whereas a l
unaffected by the magnetic fields which can cause problems when investigating certain specimens, whereas a light microscope is unaffected
fields.
Conclusion:
• Overall I believe the electron microscope is better than the light microscope to an extent; in terms of greater resolution and greater magnific
1,000,000x. This is extremely greater than the magnification of a light microscope which is only a poor 1000-1500x compared to the electron
However in terms of price, colour image and simple preparations, the light microscope wins by a mile. This is because it is in colour image, a
prepared by anyone which boosts its simplicity factors. It is also significantly cheaper than electron microscopes which are very expensive. Ta
consideration, I believe both microscopes are excellent for their own particular features and factors.
Bibliography:
• www.slideshare.net/samithsenadeera/comparison-of-light-and-electron-microscopes
