WGU biochem| 77 QUESTIONS| WITH COMPLETE SOLUTIONS

PCR- know the steps Correct Answer: 1) denaturation: heated to 95*C to separate it*** 2) annealing (cool to 50*C for sticking) 3) elongation (reaction heated to 70*C to build new DNA) ***ligase is NOT involved Ingredients for PCR: Correct Answer: Template DNA Nucleotides (dNTPs) DNA Polymerase DNA primers Base excision repair Correct Answer: how you're repairing a mutation remove damaged base and replace it DNA glycosylase see's damaged DNA and removes it DNA polymerase puts the right base back DNA lipase seals it Removes SINGLE nucleotide Nucleotide excision repair Correct Answer: Tide--beach--sun exposure= UV damage repair DNA polymerase replaces MULTIPLE BAD bases Mismatch repair Correct Answer: only one during REPLICATION- during PROOFREADING DNA polymerase proofreads MMR removes a LARGE section from DNA and DNA polymerase tries again-- repairs G-C A-T Homologous Recombination Correct Answer: repairs double stranded breaks repair is made with copy of other strands and replacing completely Nonhomologous Recombination Correct Answer: double stranded break repair cells put ends together before making sure correctly copied-- can lead to deletions/ insertions (Frameshift mutation)-- last ditch effort and body is willing to chance it DNA polymerase binds to DNA to make RNA Correct Answer: TRANSCRIPTION DNA polymerase is required for Correct Answer: DNA replication Nonsense mutation Correct Answer: Stops codon and nonsense Silent mutation Correct Answer: Changes 1